409-Ichimura-AMATERAS/Fig5_Brain_2fold
Author reviewedZ-series images of mouse brain stained with SYTOX-Green
Project
Dataset ID
Fig5_Brain_2fold
Kind
Image data
Title
Z-series images of mouse brain stained with SYTOX-Green
Description
Z-series SYTOX-Green images of mouse brain obtained by AMATERAS2. The colonally cutted 1.5 mm-thick brain section was chemically cleared using CUBIC, and the cell nuclei were stained with SYTOX-Green. The images were obtained with 2x magnification system, and the background intensity was removed by the computational sectioning.
Folder Name
Fig5_Brain_2fold
File Format
.tif
File Size
46.1GB
File Separation Method
-
Figure Number
Fig5
Dimensions
13264x9180x378x1x1
X Scale
1.1 micrometer
Y Scale
1.1 micrometer
Z Scale
4 micrometer
T Scale
-
Channel
1 channel
Quantitative Data
-
Biosample
Description
The fixed and cleared mouse brain block followed by staining with SYTOX-Green.
Organism
Mus musculus (NCBITaxon:10090)
Strain
C57BL/6J (https://www.jax.org/strain/000664)
Cell
-
Cell line
-
Intrinsic Variable
-
Extrinsic Variable
-
Ontology
Anatomical Entity
-
UBERON
brain (UBERON:0000955)
Biological Process
-
Cellular Component
nucleus (GO:0005634)
Molecular Function
-
Genetics
Gene
-
Protein / Probe
-SYTOX Green
Method / Genotype
-
Treatments
Reagent / Compound
-
Concentration
-
Fold Dilution
-
Imaging Method
Imaging Method
spinning disk confocal microscopy (FBbi:00000253)
AMATERAS-2c
Detection Method
complementary metal oxide semiconductor (CMOS)
Visualization Method
SYTOX Green
Illumination Method
-
Source of Contrast
-
Imaged Parameter
fluorescence emission
Sample Preparation Method
formaldehyde fixed tissue
Instrument
Body
custom-made
Module
-
Light Source
Thorlabs SOLIS-470C
Detector
CIS, VCC-120CXP1M
Objective
custom-made
Filter Set
Edmund Optics, #87–800 and #86–992
Dichroic
custom-made (cut-off: 497 nm)