409-Ichimura-AMATERAS/Fig2CD_CardiacSheet thumbnail

409-Ichimura-AMATERAS/Fig2CD_CardiacSheet

Author reviewed
Fluorescence image of cardiomyocytes by AMATERAS2
Project
Dataset ID
Fig2CD_CardiacSheet
Kind
Image data
Title
Fluorescence image of cardiomyocytes by AMATERAS2
Description
Fluorescence images of cardiomyocytes obtained by AMATERAS2 (A Multi-scale/modal Analytical Tool for Every Rare Activity in Singularity2). The cardiomyocytes was derived from human induced pluripotent stem cells stained with Rhodamine phalloidin.
Folder Name
Fig2CD_CardiacSheet
File Format
.tif
File Size
235MB
File Separation Method
-
Figure Number
Fig2
Dimensions
19568x12588x1x1x1
X Scale
0.75 micrometer
Y Scale
0.75 micrometer
Z Scale
-
T Scale
-
Channel
1 channel
Quantitative Data
-

Biosample

Description
Cardiomyocytes derived from human induced pluripotent stem cells stained with Rhodamine phalloidin.
Organism
Homo sapiens (NCBITaxon:9606)
Strain
-
Cell
pluripotent stem cell, human induced pluripotent stem cells (hiPSCs) (CL:0002248)
Cell line
hiPSC line 201B7
MeSH
Induced Pluripotent Stem Cells (D057026)
Microscopy, Fluorescence (D008856)
Actin Cytoskeleton (D008841)
Intrinsic Variable
-
Extrinsic Variable
-

Ontology

Anatomical Entity
-
UBERON
-
Biological Process
-
Cellular Component
actin cytoskeleton (GO:0015629)
Molecular Function
-
MeSH Anatomy
Induced Pluripotent Stem Cells (D057026)
Microscopy, Fluorescence (D008856)
Actin Cytoskeleton (D008841)
MeSH BP
Induced Pluripotent Stem Cells (D057026)
Microscopy, Fluorescence (D008856)
Actin Cytoskeleton (D008841)
MeSH CC
Induced Pluripotent Stem Cells (D057026)
Microscopy, Fluorescence (D008856)
Actin Cytoskeleton (D008841)
MeSH MF
Induced Pluripotent Stem Cells (D057026)
Microscopy, Fluorescence (D008856)
Actin Cytoskeleton (D008841)

Genetics

Gene
-
Protein / Probe
-phalloidin
Method / Genotype
-

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
fluorescence microscopy (FBbi:00000246)
AMATERAS-2w
Detection Method
complementary metal oxide semiconductor (CMOS)
Visualization Method
Rhodamine
Illumination Method
widefield illumination
Source of Contrast
-
Imaged Parameter
fluorescence emission
Sample Preparation Method
formaldehyde fixed tissue
MeSH
Induced Pluripotent Stem Cells (D057026)
Microscopy, Fluorescence (D008856)
Actin Cytoskeleton (D008841)

Instrument

Body
custom-made
Module
-
Light Source
Thorlabs SOLIS-470C
Detector
CIS, VCC-250CXP1M
Objective
custom-made
Filter Set
Edmund Optics, #87–800 and #86–992
Dichroic
custom-made (cut-off: 497 nm)