409-Ichimura-AMATERAS/Fig4_Hoechst
Author reviewedZ-series images of myocardial organoids stained with Hoechst33342
Project
Dataset ID
Fig4_Hoechst
Kind
Image data
Title
Z-series images of myocardial organoids stained with Hoechst33342
Description
Z-series images of myocardial organoids by AMATERAS2. The organoid was stained with Hoechst33342 and immunostained with troponin T. This dataset includes the images of Hoechst33342 The data of immunostaining with troponin T is reposited in another dataset, Fig4_Troponin.
Folder Name
Fig4_Hoechst
File Format
.tif
File Size
37.4GB
File Separation Method
-
Figure Number
Fig4
Dimensions
19568x12588x75x1x1
X Scale
0.75 micrometer
Y Scale
0.75 micrometer
Z Scale
4 micrometer
T Scale
-
Channel
1 channel
Quantitative Data
-
Biosample
Description
Myocardial organoids derived from hiPSC stained with anti-cardiac troponin T with Alexa488, and Hoechst33342.
Organism
Homo sapiens (NCBITaxon:9606)
Strain
-
Cell
pluripotent stem cell,
human induced pluripotent stem cells (hiPSCs) (CL:0002248)
Cell line
hiPSC line 201B7
Intrinsic Variable
-
Extrinsic Variable
-
Ontology
Anatomical Entity
-
UBERON
-
Biological Process
-
Cellular Component
nucleus (GO:0005634)
Molecular Function
-
MeSH Anatomy
MeSH BP
MeSH CC
MeSH MF
Genetics
Gene
-
Protein / Probe
-Hoechst33342
Method / Genotype
-
Treatments
Reagent / Compound
-
Concentration
-
Fold Dilution
-
Imaging Method
Imaging Method
fluorescence microscopy (FBbi:00000246)
AMATERAS-2w
Detection Method
complementary metal oxide semiconductor (CMOS)
Visualization Method
Hoechst 33342
Illumination Method
widefield illumination
Source of Contrast
-
Imaged Parameter
fluorescence emission
Sample Preparation Method
formaldehyde fixed tissue
Instrument
Body
custom-made
Module
-
Light Source
Thorlabs SOLIS-385C
Detector
CIS, VCC-250CXP1M
Objective
custom-made
Filter Set
Edmund Optics, #87–800 and #86–992
Dichroic
custom-made (cut-off: 497 nm)