409-Ichimura-AMATERAS/Fig4_Troponin
Author reviewedZ-series images of myocardial organoids stained with troponin T
Project
Dataset ID
Fig4_Troponin
Kind
Image data
Title
Z-series images of myocardial organoids stained with troponin T
Description
Z-series images of myocardial organoids by AMATERAS2. The organoid was stained with Hoechst33342 and immunostained with troponin T. This dataset includes the images of troponin T immunofluorescence. The data of Hoechst33342 is reposited in another dataset, Hoechst33342.
Folder Name
Fig4_Troponin
File Format
.tif
File Size
37.4GB
File Separation Method
-
Figure Number
Fig4
Dimensions
19568x12588x75x1x1
X Scale
0.75 micrometer
Y Scale
0.75 micrometer
Z Scale
4 micrometer
T Scale
-
Channel
1 channel
Quantitative Data
-
Biosample
Description
Myocardial organoids derived from hiPSC stained with anti-cardiac troponin T with Alexa488, and with Hoechst33342.
Organism
Homo sapiens (NCBITaxon:9606)
Strain
-
Cell
pluripotent stem cell,
human induced pluripotent stem cells (hiPSCs) (CL:0002248)
Cell line
hiPSC line 201B7
MeSH
Intrinsic Variable
-
Extrinsic Variable
-
Ontology
Anatomical Entity
-
UBERON
-
Biological Process
-
Cellular Component
actin cytoskeleton (GO:0015629)
Molecular Function
-
MeSH Anatomy
MeSH BP
MeSH CC
MeSH MF
Genetics
Gene
-
Protein / Probe
Troponin T, cardiac muscle / antibody
Method / Genotype
-
Treatments
Reagent / Compound
-
Concentration
-
Fold Dilution
-
Imaging Method
Imaging Method
fluorescence microscopy (FBbi:00000246)
AMATERAS-2w
Detection Method
complementary metal oxide semiconductor (CMOS)
Visualization Method
Alexa Fluor 488
Illumination Method
widefield illumination
Source of Contrast
-
Imaged Parameter
fluorescence emission
Sample Preparation Method
formaldehyde fixed tissue
MeSH
Instrument
Body
custom-made
Module
-
Light Source
Thorlabs SOLIS-470C
Detector
CIS, VCC-250CXP1M
Objective
custom-made
Filter Set
Edmund Optics, #87–800 and #86–992
Dichroic
custom-made (cut-off: 497 nm)