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409-Ichimura-AMATERAS/Fig6_QuailEmbyo thumbnail

409-Ichimura-AMATERAS/Fig6_QuailEmbyo

Author reviewed
Z-series and time-lapse images of the nuclei of vascular endothelial cells in quail embryo
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Project
409-Ichimura-AMATERAS
Dataset ID
Fig6_QuailEmbyo
Kind
Image data
Title
Z-series and time-lapse images of the nuclei of vascular endothelial cells in quail embryo
Description
Z-series and time-lapse images of the nuclei of vascular endothelial cells in quail embryo. The nuclei were visualized with eYFP. The images were obtained by AMATERAS2, and the background intensity was removed by the computational sectioning.
Folder Name
Fig6_QuailEmbyo
File Format
.tif
File Size
512GB
File Separation Method
-
Figure Number
Fig6
Dimensions
13264x9180x21x1x199
X Scale
1.1 micrometer
Y Scale
1.1 micrometer
Z Scale
12 micrometer
T Scale
7.5 minutes
Channel
1 channel
Quantitative Data
-

Biosample

Description
Ex ovo culture of of tie1:H2B-eYFP transgenic quail embryo in which enhanced yellow fluorescent protein visualizes the nuclei of vascular endothelial cells.
Organism
Coturnix japonica (NCBITaxon:93934)
Strain
-
Cell
endothelial cell of vascular tree (CL:0002139)
Cell line
-
MeSH
Endothelial Cells (D042783)
Microscopy, Fluorescence (D008856)
Blood Vessels (D001808)
Embryonic Development (D047108)
Cell Nucleus (D002467)
Embryonic Structures (D004628)
Cell Movement (D002465)
Intrinsic Variable
tie1:H2B-eYFP transgenic quail embryo
Extrinsic Variable
-

Ontology

Anatomical Entity
-
UBERON
embryo (UBERON:0000922)
vascular system (UBERON:0007798)
Biological Process
embryo development (GO:0009790)
cell migration (GO:0016477)
Cellular Component
nucleus (GO:0005634)
Molecular Function
-
MeSH Anatomy
Endothelial Cells (D042783)
Microscopy, Fluorescence (D008856)
Blood Vessels (D001808)
Embryonic Development (D047108)
Cell Nucleus (D002467)
Embryonic Structures (D004628)
Cell Movement (D002465)
MeSH BP
Endothelial Cells (D042783)
Microscopy, Fluorescence (D008856)
Blood Vessels (D001808)
Embryonic Development (D047108)
Cell Nucleus (D002467)
Embryonic Structures (D004628)
Cell Movement (D002465)
MeSH CC
Endothelial Cells (D042783)
Microscopy, Fluorescence (D008856)
Blood Vessels (D001808)
Embryonic Development (D047108)
Cell Nucleus (D002467)
Embryonic Structures (D004628)
Cell Movement (D002465)
MeSH MF
Endothelial Cells (D042783)
Microscopy, Fluorescence (D008856)
Blood Vessels (D001808)
Embryonic Development (D047108)
Cell Nucleus (D002467)
Embryonic Structures (D004628)
Cell Movement (D002465)

Genetics

Gene
-
Protein / Probe
Histone H2B
Method / Genotype
transgenic / Tg (tie1:H2B-eYFP)

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
fluorescence microscopy (FBbi:00000246)
AMATERAS-2w
Detection Method
complementary metal oxide semiconductor (CMOS)
Visualization Method
EYFP
Illumination Method
widefield illumination
Source of Contrast
-
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
MeSH
Endothelial Cells (D042783)
Microscopy, Fluorescence (D008856)
Blood Vessels (D001808)
Embryonic Development (D047108)
Cell Nucleus (D002467)
Embryonic Structures (D004628)
Cell Movement (D002465)

Instrument

Body
custom-made
Module
-
Light Source
Thorlabs SOLIS-470C
Detector
CIS, VCC-120CXP1M
Objective
custom-made
Filter Set
Edmund Optics, #87–800 and #86–992
Dichroic
custom-made (cut-off: 497 nm)