309-Fujioka-RamanProbe/Fig5d_FatBody_betaGal thumbnail

309-Fujioka-RamanProbe/Fig5d_FatBody_betaGal

Author reviewed
Fluorescence image of membrane and cytosolic GFP and stimulated Raman scattering (SRS) image of beta- galactosidase activity in Drosophila fat body
Project
Dataset ID
Fig5d_FatBody_betaGal
Kind
Image data
Title
Fluorescence image of membrane and cytosolic GFP and stimulated Raman scattering (SRS) image of beta- galactosidase activity in Drosophila fat body
Description
A Drosophila fat body (genotype: hs-Flp122, UAS-mCD8-GFP; Ay-Gal4, UAS-GFP/UAS-lacZ), in which beta-galactosidase (coded by lacZ) GFP are co-expressed, was incubated with 9C^{15}N-JCR-Bn-betaGal in Schneider’s Drosophila medium containing 0.5% DMSO as a cosolvent for 3.5 h at room temperature. SRS signal was detected only in LacZ-positive cells.
File or Folder
Fig5d_FatBody_betaGal
File Formats
.tif
File Separation Method
-
Source
-
X-scale
0.16 micrometer/pixel
Y-scale
0.16 micrometer/pixel
Z-scale
-
T-scale
-

Organism
Drosophila ( NCBITaxon:7215 )
Strain
-
Cell line
-
Biological Process
-
Cellular Component
-
Medical Subject Headings
-
Method involved in biological imaging
fluorescence microscopy ( FBbi:00000246 )
stimulated Raman scattering (SRS) imaging

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