SSBD:database

Key Organism Strain Cell Line Gene Symbol Protein name Protein tag Reporter Project ID Dataset ID Title Description Data Type Gene Ontology Biological Imaging Methods Method Summary Paper Information Medical Subject Headlines Person

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487-Tasaka-SMTmodulation

https://ssbd.riken.jp/database/487-Tasaka-SMTmodulation/

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ssbd-repos-000487

https://ssbd.riken.jp/repository/487/

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Submedius Thalamic Inputs Shape the Presynaptic Architecture of Layer 5 Orbitofrontal Cortex in Mice

The orbitofrontal cortex (OFC) is central to cognitive and social functions, yet its presynaptic partners remain incompletely defined. In female mice, the OFC modulates infant-directed caregiving behaviors in an experience-dependent manner. Here, we identify the submedius thalamus (SMT) as a major presynaptic partner of the OFC. Trans-synaptic tracing revealed intensive inputs from both the SMT and mediodorsal thalamus (MD) to OFC layer 5 excitatory neurons. We generated a Tnnt1-Cre mouse line that enables selective targeting of these higher-order thalamic nuclei. Axonal tracing demonstrated complementary projection patterns of the SMT and MD across prefrontal regions. Microendoscopic Ca2+ imaging demonstrated pup retrieval-related activity in both SMT and MD, with SMT exhibiting learning-related plasticity. Projection-specific chemogenetic silencing demonstrated that SMT modulates OFC activity during pup retrieval, although its behavioral relevance appeared limited. Collectively, our study provides a presynaptic map of OFC layer 5 neurons and a proof of concept for selective manipulation of the SMT as a previously understudied thalamic input to the OFC.

2026-07-09

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CC BY 4.0

Image data based on Experiment

19 ( Image datasets: 19, Quantitative data datasets: 0 )

171.4 GB ( Image datasets: 171.4 GB, Quantitative data datasets: 0 bytes )

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Mus musculus

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fluorescence microscopy (FBbi_00000246) Microendoscopic calcium imaging

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Gen-Ichi Tasaka, Mitsue Hagihara, Haruna Kobayashi, Miho Kihara, Takaya Abe, Kazunari Miyamichi (2026) Submedius thalamic inputs shape the presynaptic architecture of layer 5 orbitofrontal cortex in mice., iScience, Volume 29, Number 2, pp. 114828

Published in 2026 Feb 20 (Electronic publication in Jan. 29, 2026, midnight )

• doi: 10.1016/j.isci.2026.114828
• pmid: 41716988
  

(Abstract) The orbitofrontal cortex (OFC) is central to cognitive and social functions, yet its presynaptic partners remain incompletely defined. In female mice, the OFC modulates infant-directed caregiving behaviors in an experience-dependent manner. Here, we identify the submedius thalamus (SMT) as a major presynaptic partner of the OFC. Trans-synaptic tracing revealed intensive inputs from both the SMT and mediodorsal thalamus (MD) to OFC layer 5 excitatory neurons. We generated a Tnnt1-Cre mouse line that enables selective targeting of these higher-order thalamic nuclei. Axonal tracing demonstrated complementary projection patterns of the SMT and MD across prefrontal regions. Microendoscopic Ca(2+) imaging demonstrated pup retrieval-related activity in both SMT and MD, with SMT exhibiting learning-related plasticity. Projection-specific chemogenetic silencing demonstrated that SMT modulates OFC activity during pup retrieval, although its behavioral relevance appeared limited. Collectively, our study provides a presynaptic map of OFC layer 5 neurons and a proof of concept for selective manipulation of the SMT as a previously understudied thalamic input to the OFC.

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Kazunari Miyamichi , RIKEN BDR

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