Detail of Fig4_Tnnt1_Ai162_MD_F10_220608_AP1
Project
SSBD:Repository
Title
Time-lapse calcium imaging showing the neural activity in MD of female mouse at alloparental day 1
Description
The authors investigated the neural activity of the mediodorsal thalamus (MD) during the parental behavior of retrieving pups at two maternal stages: AP1 (alloparental day 1), AP2 (alloparental day 2). A virgin female showing more than ten pup-retrievals across two 6-min imaging sessions for the first day was defined as AP1 and the day following AP1 was defined as AP2. At AP1 and AP2, the pup-retrievals were examined using pups born from another female mouse. For the calcium imaging, the transgenic female mouse (Tnnt1-Cre; Ai162 mouse) expressing GCaMP6s in the MD_{Tnnt1} neurons was used. The signal of GCaMP6s was obtained through gradient refractive index (GRIN) lens (500 micrometres in diameter, 6.1 millimeter length; Inscopix) implanted above the MD. This dataset includes the imaging data at AP1. The data at AP2 obtained from the same female mouse are deposited in other datasets in this project; Fig4_Tnnt1_Ai162_MD_F10_220609_AP2
Release, Updated
2026-07-09
License
CC BY 4.0
Kind
Image data
File Formats
tiff
Data size
11.4 GB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
Cellular Component (CC)
Biological Imaging Method
fluorescence microscopy (FBbi_00000246) Microendoscopic calcium imaging
X scale
0.625 micrometer
Y scale
0.602 micrometer
Z scale
-
T scale
0.1 seconds
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Tasaka GI, Hagihara M, Kobayashi H, Kihara M, Abe T, Miyamichi K. Submedius thalamic inputs shape the presynaptic architecture of layer 5 orbitofrontal cortex in mice. iScience. 2026 Jan 29;29(2):114828.
Related paper(s)
Contact
Kazunari Miyamichi
, RIKEN BDR
Contributors
OMERO Project
Source