Detail of Fig3_Tnnt1_Ai162_SMT_F6_211125_AP1
Project
SSBD:Repository
Title
Time-lapse calcium imaging showing the neural activity in SMT of female mouse at alloparental day 1
Description
The authors investigated the neural activity of the submedius thalamus (SMT) during the parental behavior of retrieving pups at two maternal stages: AP1 (alloparental day 1), AP2 (alloparental day 2). A virgin female showing more than ten pup-retrievals across two 6-min imaging sessions for the first day was defined as AP1 and the day following AP1 was defined as AP2. At AP1 and AP2, the pup-retrievals were examined using pups born from another female mouse. For the calcium imaging, the transgenic female mouse (Tnnt1-Cre; Ai162 mouse) expressing GCaMP6s in the SMT_{Tnnt1} neurons was used. The signal of GCaMP6s was obtained through gradient refractive index (GRIN) lens (500 micrometres in diameter, 6.1 millimeter length; Inscopix) implanted above the SMT. This dataset includes the imaging data at AP1. The data at AP2 obtained from the same female mouse are deposited in other datasets in this project; Fig3_Tnnt1_Ai162_SMT_F6_211126_AP2
Release, Updated
2026-07-09
License
CC BY 4.0
Kind
Image data
File Formats
tiff
Data size
11.4 GB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
Cellular Component (CC)
Biological Imaging Method
fluorescence microscopy (FBbi_00000246) Microendoscopic calcium imaging
X scale
0.625 micrometer
Y scale
0.602 micrometer
Z scale
-
T scale
0.1 seconds
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
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Microscope model
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Detector model
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Objective model
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Filter set
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Summary of Methods
Tasaka GI, Hagihara M, Kobayashi H, Kihara M, Abe T, Miyamichi K. Submedius thalamic inputs shape the presynaptic architecture of layer 5 orbitofrontal cortex in mice. iScience. 2026 Jan 29;29(2):114828.
Related paper(s)
Gen-Ichi Tasaka, Mitsue Hagihara, Haruna Kobayashi, Miho Kihara, Takaya Abe, Kazunari Miyamichi (2026) Submedius thalamic inputs shape the presynaptic architecture of layer 5 orbitofrontal cortex in mice., iScience, Volume 29, Number 2, pp. 114828
Published in 2026 Feb 20 (Electronic publication in Jan. 29, 2026, midnight )
- doi: 10.1016/j.isci.2026.114828
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pmid: 41716988
(Abstract) The orbitofrontal cortex (OFC) is central to cognitive and social functions, yet its presynaptic partners remain incompletely defined. In female mice, the OFC modulates infant-directed caregiving behaviors in an experience-dependent manner. Here, we identify the submedius thalamus (SMT) as a major presynaptic partner of the OFC. Trans-synaptic tracing revealed intensive inputs from both the SMT and mediodorsal thalamus (MD) to OFC layer 5 excitatory neurons. We generated a Tnnt1-Cre mouse line that enables selective targeting of these higher-order thalamic nuclei. Axonal tracing demonstrated complementary projection patterns of the SMT and MD across prefrontal regions. Microendoscopic Ca(2+) imaging demonstrated pup retrieval-related activity in both SMT and MD, with SMT exhibiting learning-related plasticity. Projection-specific chemogenetic silencing demonstrated that SMT modulates OFC activity during pup retrieval, although its behavioral relevance appeared limited. Collectively, our study provides a presynaptic map of OFC layer 5 neurons and a proof of concept for selective manipulation of the SMT as a previously understudied thalamic input to the OFC.
Contact
Kazunari Miyamichi
, RIKEN BDR
Contributors
OMERO Project
Source