Summary of 227-Egoshi-RamanImaging

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Title
Raman imaging in HeLa cells treated with D-alkyne-tagged C18FAs
Description
-
Relase date
2022-11-23
Updated date
-
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
20 ( Image datasets: 20, Quantitative data datasets: 0 )
Size of Datasets
3.7 MB ( Image datasets: 3.7 MB, Quantitative data datasets: 0 bytes )

Organism(s)
Homo sapiens
Cell lines(s)
HeLa cell
Gene symbol(s)
NA
Protein name(s)
NA

Datatype
-
Molecular Function (MF)
Biological Process (BP)
Cellular Component (CC)
Biological Imaging Method
Raman microscopy
X scale
0.21 micrometer/ pixel
Y scale
0.21 micrometer/ pixel
Z scale
-
T scale
3 hours per time interval, NA

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

Syusuke Egoshi, Kosuke Dodo, Kenji Ohgane, Mikiko Sodeoka (2021) Deuteration of terminal alkynes realizes simultaneous live cell Raman imaging of similar alkyne-tagged biomolecules., Organic & biomolecular chemistry, Volume 19, Number 38, pp. 8232-8236

Published in 2021 Oct 6 (Electronic publication in Oct. 6, 2021, midnight )

(Abstract) Alkynes were employed as tags to observe small molecules in cells by Raman microscopy. Herein, simple deuteration was found to shift the vibrational frequency of the alkyne by 135 cm(-1). Two-color Raman imaging of D-alkynes and H-alkynes made it possible to distinguish between and observe similar small molecules in live cells.
(MeSH Terms)

Contact
Mikiko Sodeoka, Kosuke Dodo, Syusuke Egoshi , RIKEN, RIKEN, RIKEN , Cluster for Pioneering Research , Cluster for Pioneering Research , Cluster for Pioneering Research , Synthetic Organic Chemistry Laboratory , Synthetic Organic Chemistry Laboratory , Synthetic Organic Chemistry Laboratory
Contributors


Dataset List of 227-Egoshi-RamanImaging

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
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# 8698
Datast ID Fig3b_HeLa_ODYA_3
Dataset Kind Image data
Dataset Size 373.8 KB
4D view
SSBD:OMERO
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# 8699
Dataset Kind Image data
Dataset Size 445.6 KB
4D view
SSBD:OMERO
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# 8700
Datast ID Fig3d_HeLa_YOA_5
Dataset Kind Image data
Dataset Size 421.9 KB
4D view
SSBD:OMERO
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# 8701
Dataset Kind Image data
Dataset Size 281.2 KB
4D view
SSBD:OMERO
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# 8702
Dataset Kind Image data
Dataset Size 335.6 KB
4D view
SSBD:OMERO
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# 8703
Dataset Kind Image data
Dataset Size 360.5 KB
4D view
SSBD:OMERO
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# 8704
Dataset Kind Image data
Dataset Size 28.9 KB
4D view
SSBD:OMERO
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# 8705
Dataset Kind Image data
Dataset Size 28.9 KB
4D view
SSBD:OMERO
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# 8706
Dataset Kind Image data
Dataset Size 235.8 KB
4D view
SSBD:OMERO
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# 8707
Dataset Kind Image data
Dataset Size 181.5 KB
4D view
SSBD:OMERO
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# 8708
Dataset Kind Image data
Dataset Size 137.9 KB
4D view
SSBD:OMERO
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# 8709
Dataset Kind Image data
Dataset Size 229.5 KB
4D view
SSBD:OMERO
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# 8710
Dataset Kind Image data
Dataset Size 160.6 KB
4D view
SSBD:OMERO
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# 8711
Dataset Kind Image data
Dataset Size 216.2 KB
4D view
SSBD:OMERO
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# 8712
Dataset Kind Image data
Dataset Size 41.0 KB
4D view
SSBD:OMERO
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# 8713
Dataset Kind Image data
Dataset Size 41.0 KB
4D view
SSBD:OMERO
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# 8714
Dataset Kind Image data
Dataset Size 67.4 KB
4D view
SSBD:OMERO
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# 8715
Dataset Kind Image data
Dataset Size 67.4 KB
4D view
SSBD:OMERO
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# 8716
Dataset Kind Image data
Dataset Size 56.0 KB
4D view
SSBD:OMERO
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Download Image data

# 8717
Dataset Kind Image data
Dataset Size 56.0 KB
4D view
SSBD:OMERO
Download BDML
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