Detail of Fig5_HeLa_dual_2to1
Project
SSBD:Repository
Title
Raman images of H-alkyne and D-alkyne signals in HeLa cells treated with D-3 and 5 at 2:1 dose for 6 hours.
Description
Raman images of H-alkyne and D-alkyne signals in HeLa cells treated with D-3 and 5 at 2:1 dose for 6 hours.
Release, Updated
2022-11-23
License
CC BY
Kind
Image data
File Formats
.bmp
Data size
335.6 KB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
Cellular Component (CC)
Biological Imaging Method
Raman microscopy
X scale
0.21 micrometer/ pixel
Y scale
0.21 micrometer/ pixel
Z scale
-
T scale
-
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
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Contrast method
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Microscope model
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Detector model
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Objective model
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Filter set
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Summary of Methods
See details in Egoshi S, et. al. (2021) Org Biomol Chem, Oct 6;19(38):8232-8236.
Related paper(s)
Syusuke Egoshi, Kosuke Dodo, Kenji Ohgane, Mikiko Sodeoka (2021) Deuteration of terminal alkynes realizes simultaneous live cell Raman imaging of similar alkyne-tagged biomolecules., Organic & biomolecular chemistry, Volume 19, Number 38, pp. 8232-8236
Published in 2021 Oct 6 (Electronic publication in Oct. 6, 2021, midnight )
- doi: 10.1039/d1ob01479j
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pmid: 34528645
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url: https://pubs.rsc.org/en/content/articlelanding/2021/OB/D1OB01479J
(Abstract) Alkynes were employed as tags to observe small molecules in cells by Raman microscopy. Herein, simple deuteration was found to shift the vibrational frequency of the alkyne by 135 cm(-1). Two-color Raman imaging of D-alkynes and H-alkynes made it possible to distinguish between and observe similar small molecules in live cells.(MeSH Terms)
Contact
Mikiko Sodeoka, Kosuke Dodo, Syusuke Egoshi
, RIKEN, RIKEN, RIKEN
, Cluster for Pioneering Research , Cluster for Pioneering Research , Cluster for Pioneering Research
, Synthetic Organic Chemistry Laboratory , Synthetic Organic Chemistry Laboratory , Synthetic Organic Chemistry Laboratory
Contributors
OMERO Dataset
OMERO Project
Source