Detail of FigS8_HeLa_dual_200uM_H-alkyne



Project
SSBD:Repository
Title
Raman images of H-alkyne in HeLa cells treated with 3 and 4 at 2:1 dose ratio for 6 hours.
Description
Raman images of H-alkyne in HeLa cells treated with 3 and 4 at 2:1 dose ratio for 6 hours.
Release, Updated
2022-11-23
License
CC-BY
Kind
Image data
File Formats
.bmp
Data size
67.4 KB

Organism
Homo sapiens ( NCBITaxon:9606 )
Strain(s)
-
Cell Line
HeLa cell ( CLO_0003684 )

Datatype
-
Molecular Function (MF)
Biological Process (BP)
Cellular Component (CC)
Biological Imaging Method
Raman microscopy
X scale
0.21 micrometer/ pixel
Y scale
0.21 micrometer/ pixel
Z scale
-
T scale
-

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
See details in Egoshi S, et. al. (2021) Org Biomol Chem, Oct 6;19(38):8232-8236.
Related paper(s)

Syusuke Egoshi, Kosuke Dodo, Kenji Ohgane, Mikiko Sodeoka (2021) Deuteration of terminal alkynes realizes simultaneous live cell Raman imaging of similar alkyne-tagged biomolecules., Organic & biomolecular chemistry, Volume 19, Number 38, pp. 8232-8236

Published in 2021 Oct 6 (Electronic publication in Oct. 6, 2021, midnight )

(Abstract) Alkynes were employed as tags to observe small molecules in cells by Raman microscopy. Herein, simple deuteration was found to shift the vibrational frequency of the alkyne by 135 cm(-1). Two-color Raman imaging of D-alkynes and H-alkynes made it possible to distinguish between and observe similar small molecules in live cells.
(MeSH Terms)

Contact
Mikiko Sodeoka, Kosuke Dodo, Syusuke Egoshi , RIKEN, RIKEN, RIKEN , Cluster for Pioneering Research , Cluster for Pioneering Research , Cluster for Pioneering Research , Synthetic Organic Chemistry Laboratory , Synthetic Organic Chemistry Laboratory , Synthetic Organic Chemistry Laboratory
Contributors

OMERO Dataset
OMERO Project
Source