ssbd-repos-000227 - SSBD:repository Systems Science of Biological Dynamics repository

Summary of ssbd-repos-000227

Title

Raman imaging in HeLa cells treated with D-alkyne-tagged C18FAs

Description

Two-color Raman imaging of similar small molecules in living cells by using D-/H-alkynes as Raman tags

Submited Date

2022-04-18

Release Date

2022-04-18

Organism

H. sapiens

Molecular Function (MF)

Biological Process (BP)

fatty acids localization, EdU localization

Cellular Component (CC)

lipids, nucleus

Study Type

Development of new Raman tag

Imaging Methods

Spontaneous Raman microscopy

Method Summary

Egoshi S, Dodo K, Ohgane K, Sodeoka M. Deuteration of terminal alkynes realizes simultaneous live cell Raman imaging of similar alkyne-tagged biomolecules. Org Biomol Chem. 2021 Oct 14;19(38), 8232-8236. doi.org/10.1039/D1OB01479J. PMID: 34528645.

Related paper(s)

Syusuke Egoshi, Kosuke Dodo, Kenji Ohgane, Mikiko Sodeoka (2021) Deuteration of terminal alkynes realizes simultaneous live cell Raman imaging of similar alkyne-tagged biomolecules., Organic & biomolecular chemistry, Volume 19, Number 38, pp. 8232-8236

Published in 2021 Oct 6 (Electronic publication in Oct. 6, 2021, midnight )

doi: 10.1039/d1ob01479j
pmid: 34528645
url: https://pubs.rsc.org/en/content/articlelanding/2021/OB/D1OB01479J

(Abstract) Alkynes were employed as tags to observe small molecules in cells by Raman microscopy. Herein, simple deuteration was found to shift the vibrational frequency of the alkyne by 135 cm(-1). Two-color Raman imaging of D-alkynes and H-alkynes made it possible to distinguish between and observe similar small molecules in live cells.

(MeSH Terms)

Alkynes[major]

Contact(s)

Kosuke Dodo, Mikiko Sodeoka

Organization(s)

RIKEN , Cluster for Pioneering Research , Synthetic organic chemistry laboratory

Image Data Contributors

Syusuke Egoshi

Quantitative Data Contributors

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