SSBD:database

Summary of 227-Egoshi-RamanImaging

SSBD:database
227-Egoshi-RamanImaging
SSBD:database URL
https://ssbd.riken.jp/database/227-Egoshi-RamanImaging/
SSBD:repository
ssbd-repos-000227
SSBD:repository URL
Title
Raman imaging in HeLa cells treated with D-alkyne-tagged C18FAs
Description
-
Relase date
2022-11-23
Updated date
-
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
20 ( Image datasets: 20, Quantitative data datasets: 0 )
Size of Datasets
3.7 MB ( Image datasets: 3.7 MB, Quantitative data datasets: 0 bytes )
Organism(s)
Homo sapiens
Cell lines(s)
HeLa cell
Datatype
-
Molecular Function (MF)
Biological Process (BP)
Cellular Component (CC)
Biological Imaging Method
Raman microscopy
X scale
0.21 micrometer/ pixel
Y scale
0.21 micrometer/ pixel
Z scale
-
T scale
3 hours per time interval, NA
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Related paper(s)

Syusuke Egoshi, Kosuke Dodo, Kenji Ohgane, Mikiko Sodeoka (2021) Deuteration of terminal alkynes realizes simultaneous live cell Raman imaging of similar alkyne-tagged biomolecules., Organic & biomolecular chemistry, Volume 19, Number 38, pp. 8232-8236

Published in 2021 Oct 6 (Electronic publication in Oct. 6, 2021, midnight )

(Abstract) Alkynes were employed as tags to observe small molecules in cells by Raman microscopy. Herein, simple deuteration was found to shift the vibrational frequency of the alkyne by 135 cm(-1). Two-color Raman imaging of D-alkynes and H-alkynes made it possible to distinguish between and observe similar small molecules in live cells.
(MeSH Terms)
Contact
Mikiko Sodeoka, Kosuke Dodo, Syusuke Egoshi , RIKEN, RIKEN, RIKEN , Cluster for Pioneering Research , Cluster for Pioneering Research , Cluster for Pioneering Research , Synthetic Organic Chemistry Laboratory , Synthetic Organic Chemistry Laboratory , Synthetic Organic Chemistry Laboratory
Contributors

Dataset List of 227-Egoshi-RamanImaging

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
Download BDML
Download Images
# 8698
Datast ID Fig3b_HeLa_ODYA_3
Dataset Kind Image data
Dataset Size 373.8 KB
4D view
SSBD:OMERO
Download BDML
Download Image data
# 8699
Datast ID Fig3c_HeLa_D-ODYA_D-3
Dataset Kind Image data
Dataset Size 445.6 KB
4D view
SSBD:OMERO
Download BDML
Download Image data
# 8700
Datast ID Fig3d_HeLa_YOA_5
Dataset Kind Image data
Dataset Size 421.9 KB
4D view
SSBD:OMERO
Download BDML
Download Image data
# 8701
Datast ID Fig5_HeLa_dual_1to1
Dataset Kind Image data
Dataset Size 281.2 KB
4D view
SSBD:OMERO
Download BDML
Download Image data
# 8702
Datast ID Fig5_HeLa_dual_2to1
Dataset Kind Image data
Dataset Size 335.6 KB
4D view
SSBD:OMERO
Download BDML
Download Image data
# 8703
Datast ID Fig5_HeLa_dual_4to1
Dataset Kind Image data
Dataset Size 360.5 KB
4D view
SSBD:OMERO
Download BDML
Download Image data
# 8704
Datast ID FigS6_HeLa_dual_D-alkyne
Dataset Kind Image data
Dataset Size 28.9 KB
4D view
SSBD:OMERO
Download BDML
Download Image data
# 8705
Datast ID FigS6_HeLa_dual_H-alkyne
Dataset Kind Image data
Dataset Size 28.9 KB
4D view
SSBD:OMERO
Download BDML
Download Image data
# 8706
Datast ID FigS7_HeLa_ODYA_3_100uM
Dataset Kind Image data
Dataset Size 235.8 KB
4D view
SSBD:OMERO
Download BDML
Download Image data
# 8707
Datast ID FigS7_HeLa_ODYA_3_200uM
Dataset Kind Image data
Dataset Size 181.5 KB
4D view
SSBD:OMERO
Download BDML
Download Image data
# 8708
Datast ID FigS7_HeLa_ODYA_3_400uM
Dataset Kind Image data
Dataset Size 137.9 KB
4D view
SSBD:OMERO
Download BDML
Download Image data
# 8709
Datast ID FigS7_HeLa_ODYA_D-3_100uM
Dataset Kind Image data
Dataset Size 229.5 KB
4D view
SSBD:OMERO
Download BDML
Download Image data
# 8710
Datast ID FigS7_HeLa_ODYA_D-3_200uM
Dataset Kind Image data
Dataset Size 160.6 KB
4D view
SSBD:OMERO
Download BDML
Download Image data
# 8711
Datast ID FigS7_HeLa_ODYA_D-3_400uM
Dataset Kind Image data
Dataset Size 216.2 KB
4D view
SSBD:OMERO
Download BDML
Download Image data
# 8712
Datast ID FigS8_HeLa_dual_100uM_cis-olefin
Dataset Kind Image data
Dataset Size 41.0 KB
4D view
SSBD:OMERO
Download BDML
Download Image data
# 8713
Datast ID FigS8_HeLa_dual_100uM_H-alkyne
Dataset Kind Image data
Dataset Size 41.0 KB
4D view
SSBD:OMERO
Download BDML
Download Image data
# 8714
Datast ID FigS8_HeLa_dual_100uM_cis-olefin_2
Dataset Kind Image data
Dataset Size 67.4 KB
4D view
SSBD:OMERO
Download BDML
Download Image data
# 8715
Datast ID FigS8_HeLa_dual_200uM_H-alkyne
Dataset Kind Image data
Dataset Size 67.4 KB
4D view
SSBD:OMERO
Download BDML
Download Image data
# 8716
Datast ID FigS8_HeLa_dual_100uM_cis-olefin_3
Dataset Kind Image data
Dataset Size 56.0 KB
4D view
SSBD:OMERO
Download BDML
Download Image data
# 8717
Datast ID FigS8_HeLa_dual_400uM_H-alkyne
Dataset Kind Image data
Dataset Size 56.0 KB
4D view
SSBD:OMERO
Download BDML
Download Image data