SSBD:database

Key Organism Strain Cell Line Gene Symbol Protein name Protein tag Reporter Project ID Dataset ID Title Description Data Type Gene Ontology Biological Imaging Methods Method Summary Paper Information Medical Subject Headlines Person

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379-Okubo-bilaminoid

https://ssbd.riken.jp/database/379-Okubo-bilaminoid/

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ssbd-repos-000379

https://ssbd.riken.jp/repository/379/

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3D images of bilaminar organoid generated from co-culture of human pluripotent stem cells (hPSC) and hPSC-derived hypoblast-like cells

Recently, several studies using cultures of human embryos together with single-cell RNA-seq analyses have revealed differences between humans and mice, necessitating the study of human embryos. Despite the importance of human embryology, ethical and legal restrictions have limited post-implantation-stage studies. Thus, recent efforts have focused on developing in vitro self-organizing models using human stem cells. In this paper, the authors report genetic and non-genetic approaches to generate authentic hypoblast cells (naive hPSC-derived hypoblast-like cells (nHyCs))-known to give rise to one of the two extraembryonic tissues essential for embryonic development-from naive human pluripotent stem cells (hPSCs). Their nHyCs spontaneously assemble with naive hPSCs to form a three-dimensional bilaminar structure (bilaminoids) with a pro-amniotic-like cavity. In the presence of additional naive hPSC-derived analogues of the second extraembryonic tissue, the trophectoderm, the efficiency of bilaminoid formation increases from 20% to 40%, and the epiblast within the bilaminoids continues to develop in response to trophectoderm-secreted IL-6. Furthermore, they show that bilaminoids robustly recapitulate the patterning of the anterior-posterior axis and the formation of cells reflecting the pregastrula stage, the emergence of which can be shaped by genetically manipulating the DKK1/OTX2 hypoblast-like domain.

2025-04-21

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CC BY

Image data based on Experiment

8 ( Image datasets: 8, Quantitative data datasets: 0 )

1.9 GB ( Image datasets: 1.9 GB, Quantitative data datasets: 0 bytes )

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Homo sapiens

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embryo development

confocal microscopy

0.24 micrometer, NA, 0.104 micrometer, 0.194 micrometer, 0.181 micrometer, 0.26 micrometer

0.24 micrometer, NA, 0.104 micrometer, 0.194 micrometer, 0.181 micrometer, 0.26 micrometer

NA, 3.00 micrometer, 5.00 micrometer

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Takuya Yamamoto, Yasuhiro Takashima , Kyoto University, Kyoto University , Center for iPS Cell Research and Application,, Center for iPS Cell Research and Application, , Center for iPS Cell Research and Application,, Center for iPS Cell Research and Application,

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