Summary of 282-Ohgushi-StemCell

SSBD:database
SSBD:database URL
Title
Embryonic stem cell differentiation
Description
-
Relase date
2024-11-25
Updated date
-
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
4 ( Image datasets: 4, Quantitative data datasets: 0 )
Size of Datasets
2.5 GB ( Image datasets: 2.5 GB, Quantitative data datasets: 0 bytes )

Organism(s)
Homo sapiens
Gene symbol(s)
NA
Protein name(s)
NA

Datatype
-
Molecular Function (MF)
Biological Process (BP)
morphogenesis of an epithelium, syncytium formation, cell division, stem cell differentiation
Cellular Component (CC)
Biological Imaging Method
time lapse microscopy, fluorescence microscopy, confocal microscopy
T scale
10 minutes per time interval, 15 minutes per time interval, NA

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

Masatoshi Ohgushi, Nobuko Taniyama, Alexis Vandenbon, Mototsugu Eiraku (2022) Delamination of trophoblast-like syncytia from the amniotic ectodermal analogue in human primed embryonic stem cell-based differentiation model., Cell reports, Volume 39, Number 12, pp. 110973

Published in 2022 Jun 21

(Abstract) Human primed embryonic stem cells (ESCs) are known to be converted to cells with several trophoblast properties, but it has remained controversial whether this phenomenon represents the inherent differentiation competence of human primed ESCs to trophoblast lineages. In this study, we report that chemical blockage of ACTIVIN/NODAL and FGF signals is sufficient to steer human primed ESCs into GATA3-expressing cells that give rise to placental hormone-producing syncytia analogous to syncytiotrophoblasts of the post-implantation stage of the human embryo. Despite their cytological similarity to syncytiotrophoblasts, these syncytia arise from the non-trophoblastic differentiation trajectory that recapitulates amniogenesis. These results provide insights into the possible extraembryonic differentiation pathway that is unique in primate embryogenesis.
(MeSH Terms)

Contact
Masatoshi Ohgushi , Kyoto University , Institute for Life and Medical Sciences , Laboratory of Organoids Technology, Center for Human ES Cell Research
Contributors


Dataset List of 282-Ohgushi-StemCell

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
Download BDML
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# 11586
Datast ID VideoS1_GATA3posi
Dataset Kind Image data
Dataset Size 656.7 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 11587
Dataset Kind Image data
Dataset Size 389.3 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 11588
Datast ID Fig4A_APtreat
Dataset Kind Image data
Dataset Size 891.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 11589
Datast ID VideoS3_CellDiv
Dataset Kind Image data
Dataset Size 579.8 MB
4D view
SSBD:OMERO
Download BDML
Download Image data