Detail of VideoS2_syncytialization
Project
SSBD:Repository
Title
Time-lapse images of syncytium formation from ESCs (human primed embryonic stem cells)
Description
To monitor syncytium formation, two lines of ESC that were marked with different fluorescent proteins (H2B-Venus and mCherry, respectively) were independently treated with AP for 4 days, and then mixed and cocultured. After 4–6 days of coculture, the appearance of syncytia, defined as cells expressing both the Venus signals in the nucleus and mCherry signals in the cell body, was detected.
Release, Updated
2024-11-25
License
CC BY
Kind
Image data
File Formats
.tif
Data size
389.3 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
syncytium formation
(
GO:0006949
)
Cellular Component (CC)
Biological Imaging Method
X scale
0.8 micrometer/pixel
Y scale
0.8 micrometer/pixel
Z scale
-
T scale
10 minutes per time interval
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
See details in Ohgushi M, et. al. Cell Rep. 2022 Jun 21;39(12):110973
Related paper(s)
Contact
Masatoshi Ohgushi
, Kyoto University
, Institute for Life and Medical Sciences
, Laboratory of Organoids Technology, Center for Human ES Cell Research
Contributors
OMERO Project
Source