Detail of VideoS2_syncytialization
Project
Title
Time-lapse images of syncytium formation from ESCs (human primed embryonic stem cells)
Description
To monitor syncytium formation, two lines of ESC that were marked with different fluorescent proteins (H2B-Venus and mCherry, respectively) were independently treated with AP for 4 days, and then mixed and cocultured. After 4–6 days of coculture, the appearance of syncytia, defined as cells expressing both the Venus signals in the nucleus and mCherry signals in the cell body, was detected.
Release, Updated
2024-11-25
License
CC BY
Kind
Image data
File Formats
.tif
Data size
389.3 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
syncytium formation
(
GO:0006949
)
Cellular Component (CC)
Biological Imaging Method
X scale
0.8 micrometer/pixel
Y scale
0.8 micrometer/pixel
Z scale
-
T scale
10 minutes per time interval
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
See details in Ohgushi M, et. al. Cell Rep. 2022 Jun 21;39(12):110973
Related paper(s)
Masatoshi Ohgushi, Nobuko Taniyama, Alexis Vandenbon, Mototsugu Eiraku (2022) Delamination of trophoblast-like syncytia from the amniotic ectodermal analogue in human primed embryonic stem cell-based differentiation model., Cell reports, Volume 39, Number 12, pp. 110973
Published in 2022 Jun 21
- doi: 10.1016/j.celrep.2022.110973
-
pmid: 35732132
(Abstract) Human primed embryonic stem cells (ESCs) are known to be converted to cells with several trophoblast properties, but it has remained controversial whether this phenomenon represents the inherent differentiation competence of human primed ESCs to trophoblast lineages. In this study, we report that chemical blockage of ACTIVIN/NODAL and FGF signals is sufficient to steer human primed ESCs into GATA3-expressing cells that give rise to placental hormone-producing syncytia analogous to syncytiotrophoblasts of the post-implantation stage of the human embryo. Despite their cytological similarity to syncytiotrophoblasts, these syncytia arise from the non-trophoblastic differentiation trajectory that recapitulates amniogenesis. These results provide insights into the possible extraembryonic differentiation pathway that is unique in primate embryogenesis.(MeSH Terms)
Contact
Masatoshi Ohgushi
, Kyoto University
, Institute for Life and Medical Sciences
, Laboratory of Organoids Technology, Center for Human ES Cell Research
Contributors
OMERO Project
Source