Detail of VideoS1_GATA3posi
Project
SSBD:Repository
Title
Live cell image for ESCs (human primed embryonic stem cells) into GATA3-expressing cells
Description
Live imaging of the GATA3-knockin KhES-1 cells treated with AP for 72 h. AP treatment is addition of the two inhibitors for the ACTIVIN/NODAL and FGF signaling. Recording was started immediately after AP addition. G2-4 DIC.tif is images for bright field, and G2-4 Red.tif is images for red fluorescence (The fluorescence of tandem tomato). The fluorescence indicates GATA3-expressing cells. Timestamp is attached on the images.
Release, Updated
2024-11-25
License
CC BY
Kind
Image data
File Formats
.tif
Data size
656.7 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
stem cell differentiation
(
GO:0048863
)
Cellular Component (CC)
Biological Imaging Method
X scale
3.2 micrometer/pixel
Y scale
3.2 micrometer/pixel
Z scale
-
T scale
10 minutes per time interval
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
See details in Ohgushi M, et. al. Cell Rep. 2022 Jun 21;39(12):110973
Related paper(s)
Contact
Masatoshi Ohgushi
, Kyoto University
, Institute for Life and Medical Sciences
, Laboratory of Organoids Technology, Center for Human ES Cell Research
Contributors
OMERO Project