Summary of 222-Dodo-RamanImaging
Title
Raman images of VA-13 cells or WI-38 cells treated with 100 µM all-D-GLA at different time point
Kind
Image data
based on Experiment
Number of Datasets
12
( Image datasets: 12,
Quantitative data datasets: 0 )
Size of Datasets
4.6 MB
( Image datasets: 4.6 MB,
Quantitative data datasets: 0 bytes )
Cell lines(s)
WI-38 cell, Wi-38 cell, VA-13 cell
Biological Process (BP)
metabolic process
Biological Imaging Method
Raman microscopy
X scale
0.21 micrometer/ pixel
Y scale
0.21 micrometer/ pixel
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Related paper(s)
Kosuke Dodo, Ayato Sato, Yuki Tamura, Syusuke Egoshi, Koichi Fujiwara, Kana Oonuma, Shuhei Nakao, Naoki Terayama, Mikiko Sodeoka (2021) Synthesis of deuterated gamma-linolenic acid and application for biological studies: metabolic tuning and Raman imaging., Chemical communications (Cambridge, England), Volume 57, Number 17, pp. 2180-2183
Published in 2021 Mar 1
(Abstract) gamma-Linolenic acid (GLA) is reported to show tumor-selective cytotoxicity through unidentified mechanisms. Here, to assess the involvement of oxidized metabolites of GLA, we synthesized several deuterated GLAs and evaluated their metabolism and cytotoxicity towards normal human fibroblast WI-38 cells and VA-13 tumor cells generated from WI-38 by transformation with SV40 virus. Deuteration of GLA suppressed both metabolism and cytotoxicity towards WI-38 cells and increased the selectivity for VA-13 cells. Fully deuterated GLA was visualized by Raman imaging, which indicated that GLA is accumulated in intracellular lipid droplets of VA-13 cells. Our results suggest the tumor-selective cytotoxicity is due to GLA itself, not its oxidized metabolites.
(MeSH Terms)
Contact
Mikiko Sodeoka, Kosuke Dodo, Syusuke Egoshi
, RIKEN, RIKEN, RIKEN
, Cluster for Pioneering Research , Cluster for Pioneering Research , Cluster for Pioneering Research
, Synthetic Organic Chemistry Laboratory , Synthetic Organic Chemistry Laboratory , Synthetic Organic Chemistry Laboratory
SSBD:OMERO.gallery Project
SSBD:OMERO Project
Dataset List of 222-Dodo-RamanImaging
#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
Download BDML
Download Images
Dataset Kind
Image data
Dataset Size
359.2 KB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (158.1 KB)
Dataset Kind
Image data
Dataset Size
410.5 KB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (180.9 KB)
Dataset Kind
Image data
Dataset Size
304.2 KB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (169.4 KB)
Dataset Kind
Image data
Dataset Size
459.2 KB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (276.1 KB)
Dataset Kind
Image data
Dataset Size
315.1 KB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (180.7 KB)
Dataset Kind
Image data
Dataset Size
415.2 KB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (232.4 KB)
Dataset Kind
Image data
Dataset Size
537.0 KB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (309.1 KB)
Dataset Kind
Image data
Dataset Size
506.2 KB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (300.6 KB)
Dataset Kind
Image data
Dataset Size
501.4 KB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (287.8 KB)
Dataset Kind
Image data
Dataset Size
446.5 KB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (270.9 KB)
Dataset Kind
Image data
Dataset Size
270.9 KB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (98.6 KB)
Dataset Kind
Image data
Dataset Size
208.4 KB
4D view
4D View
SSBD:OMERO
gallery
index
Download BDML
zip
Download Image data
zip (81.3 KB)