Detail of Fig6_S4_Raman_imaging_WI-38cell_12h
Project
SSBD:Repository
Title
Raman images of WI-38 cells treated with 100 µM all-D-GLA at the time point of 12 hours.
Description
Raman images of WI-38 cells treated with 100 µM all-D-GLA at the time point of 12 hours.
Release, Updated
2022-11-23
License
CC BY-NC
Kind
Image data
File Formats
.bmp
Data size
506.2 KB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
metabolic process
(
GO:0008152
)
Cellular Component (CC)
Biological Imaging Method
Raman microscopy
X scale
0.21 micrometer/ pixel
Y scale
0.21 micrometer/ pixel
Z scale
-
T scale
-
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
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Objective model
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Filter set
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Summary of Methods
See details in Dodo K, et. al. (2021) Chem Commun (Camb), Mar 1;57(17):2180-2183.
Related paper(s)
Kosuke Dodo, Ayato Sato, Yuki Tamura, Syusuke Egoshi, Koichi Fujiwara, Kana Oonuma, Shuhei Nakao, Naoki Terayama, Mikiko Sodeoka (2021) Synthesis of deuterated gamma-linolenic acid and application for biological studies: metabolic tuning and Raman imaging., Chemical communications (Cambridge, England), Volume 57, Number 17, pp. 2180-2183
Published in 2021 Mar 1
- doi: 10.1039/d0cc07824g
-
pmid: 33527102
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url: https://pubs.rsc.org/en/content/articlelanding/2021/CC/D0CC07824G
(Abstract) gamma-Linolenic acid (GLA) is reported to show tumor-selective cytotoxicity through unidentified mechanisms. Here, to assess the involvement of oxidized metabolites of GLA, we synthesized several deuterated GLAs and evaluated their metabolism and cytotoxicity towards normal human fibroblast WI-38 cells and VA-13 tumor cells generated from WI-38 by transformation with SV40 virus. Deuteration of GLA suppressed both metabolism and cytotoxicity towards WI-38 cells and increased the selectivity for VA-13 cells. Fully deuterated GLA was visualized by Raman imaging, which indicated that GLA is accumulated in intracellular lipid droplets of VA-13 cells. Our results suggest the tumor-selective cytotoxicity is due to GLA itself, not its oxidized metabolites.(MeSH Terms)
Contact
Mikiko Sodeoka, Kosuke Dodo, Syusuke Egoshi
, RIKEN, RIKEN, RIKEN
, Cluster for Pioneering Research , Cluster for Pioneering Research , Cluster for Pioneering Research
, Synthetic Organic Chemistry Laboratory , Synthetic Organic Chemistry Laboratory , Synthetic Organic Chemistry Laboratory
Contributors
OMERO Dataset
OMERO Project
Source