Summary of 222-Dodo-RamanImaging

SSBD:database
SSBD:database URL

SSBD:repository
SSBD:repository URL

Title
Raman images of VA-13 cells or WI-38 cells treated with 100 µM all-D-GLA at different time point
Description
-
Relase date
2022-11-23
Updated date
-
License
CC BY-NC
Kind
Image data based on Experiment
Number of Datasets
12 ( Image datasets: 12, Quantitative data datasets: 0 )
Size of Datasets
4.6 MB ( Image datasets: 4.6 MB, Quantitative data datasets: 0 bytes )

Organism(s)
Homo sapiens
Cell lines(s)
WI-38 cell, Wi-38 cell, VA-13 cell
Gene symbol(s)
NA
Protein name(s)
NA

Datatype
-
Molecular Function (MF)
Biological Process (BP)
metabolic process
Cellular Component (CC)
Biological Imaging Method
Raman microscopy
X scale
0.21 micrometer/ pixel
Y scale
0.21 micrometer/ pixel
Z scale
-
T scale
-

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

Kosuke Dodo, Ayato Sato, Yuki Tamura, Syusuke Egoshi, Koichi Fujiwara, Kana Oonuma, Shuhei Nakao, Naoki Terayama, Mikiko Sodeoka (2021) Synthesis of deuterated gamma-linolenic acid and application for biological studies: metabolic tuning and Raman imaging., Chemical communications (Cambridge, England), Volume 57, Number 17, pp. 2180-2183

Published in 2021 Mar 1

(Abstract) gamma-Linolenic acid (GLA) is reported to show tumor-selective cytotoxicity through unidentified mechanisms. Here, to assess the involvement of oxidized metabolites of GLA, we synthesized several deuterated GLAs and evaluated their metabolism and cytotoxicity towards normal human fibroblast WI-38 cells and VA-13 tumor cells generated from WI-38 by transformation with SV40 virus. Deuteration of GLA suppressed both metabolism and cytotoxicity towards WI-38 cells and increased the selectivity for VA-13 cells. Fully deuterated GLA was visualized by Raman imaging, which indicated that GLA is accumulated in intracellular lipid droplets of VA-13 cells. Our results suggest the tumor-selective cytotoxicity is due to GLA itself, not its oxidized metabolites.
(MeSH Terms)

Contact
Mikiko Sodeoka, Kosuke Dodo, Syusuke Egoshi , RIKEN, RIKEN, RIKEN , Cluster for Pioneering Research , Cluster for Pioneering Research , Cluster for Pioneering Research , Synthetic Organic Chemistry Laboratory , Synthetic Organic Chemistry Laboratory , Synthetic Organic Chemistry Laboratory
Contributors


Dataset List of 222-Dodo-RamanImaging

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
Download BDML
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# 7773
Dataset Kind Image data
Dataset Size 359.2 KB
4D view
SSBD:OMERO
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# 7774
Dataset Kind Image data
Dataset Size 410.5 KB
4D view
SSBD:OMERO
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# 7775
Dataset Kind Image data
Dataset Size 304.2 KB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 7776
Dataset Kind Image data
Dataset Size 459.2 KB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 7777
Dataset Kind Image data
Dataset Size 315.1 KB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 7778
Dataset Kind Image data
Dataset Size 415.2 KB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 7779
Dataset Kind Image data
Dataset Size 537.0 KB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 7780
Dataset Kind Image data
Dataset Size 506.2 KB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 7781
Dataset Kind Image data
Dataset Size 501.4 KB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 7782
Dataset Kind Image data
Dataset Size 446.5 KB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 7783
Dataset Kind Image data
Dataset Size 270.9 KB
4D view
SSBD:OMERO
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# 7784
Dataset Kind Image data
Dataset Size 208.4 KB
4D view
SSBD:OMERO
Download BDML
Download Image data