SSBD:database

Kohei Asai, Yuanzhuo Zhou, Osamu Takenouchi, Tomoya S Kitajima (2024) Artificial kinetochore beads establish a biorientation-like state in the spindle., Science (New York, N.Y.), Volume 385, Number 6715, pp. 1366-1375

Published in 2024 Sep 20 (Electronic publication in Sept. 19, 2024, midnight )

• doi: 10.1126/science.adn5428
• pmid: 39298589
  

(Abstract) Faithful chromosome segregation requires biorientation, where the pair of kinetochores on the chromosome establish bipolar microtubule attachment. The integrity of the kinetochore, a macromolecular complex built on centromeric DNA, is required for biorientation, but components sufficient for biorientation remain unknown. Here, we show that tethering the outer kinetochore heterodimer NDC80-NUF2 to the surface of apolar microbeads establishes their biorientation-like state in mouse cells. NDC80-NUF2 microbeads align at the spindle equator and self-correct alignment errors. The alignment is associated with stable bipolar microtubule attachment and is independent of the outer kinetochore proteins SPC24-SPC25, KNL1, the Mis12 complex, inner kinetochore proteins, and Aurora. Larger microbeads align more rapidly, suggesting a size-dependent biorientation mechanism. This study demonstrates a biohybrid kinetochore design for synthetic biorientation of microscale particles in cells.

• Animals
• Mice
• Cell Cycle Proteins/metabolism[major]
• Chromosome Segregation[major]
• Cytoskeletal Proteins/metabolism
• Cytoskeletal Proteins/genetics
• Kinetochores/metabolism[major]
• Microspheres[major]
• Microtubule-Associated Proteins/metabolism[major]
• Microtubule-Associated Proteins/genetics[major]
• Microtubules/metabolism[major]
• Nuclear Proteins/metabolism
• Nuclear Proteins/genetics
• Spindle Apparatus/metabolism[major]