SSBD:database

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Detail of Fig1C_Chr-and-beads_14

Project

422-Asai-KinetochoreBeads

SSBD:Repository

ssbd-repos-000422

Title

Z-series and time-lapse images of chromosomes and NDC80-NUF2 beads in an oocyte

Description

Z-series and time-lapse images of chromosomes and NDC80-NUF2 beads in a mouse oocyte. NDC80-NUF2 (NDC80-NUF2 heterodimer) beads were formed in the oocyte as follows; The authors microinjected mRNAs encoding C-terminally green fluorescent protein–tagged NDC80 (NDC80-GFP) and NUF2, followed by anti-GFP–conjugated microbeads into the cytoplasm. The mRNA for H2B-mCherry was also injected to visualize the chromosomes. channel1; GFP, channel2; mCherry, channel3;bright field.

Release, Updated

2025-11-28

License

CC BY 4.0

Kind

Image data

File Formats

.lsm

Data size

5.3 GB

Organism

Mus musculus ( NCBI:txid10090 )

Strain(s)

B6D2F1

Cell Line

Datatype

Molecular Function (MF)

Biological Process (BP)

female meiosis chromosome segregation , kinetochore assembly

Cellular Component (CC)

kinetochore , chromosome

Biological Imaging Method

confocal microscopy ( Fbbi:00000251 )
time lapse microscopy ( Fbbi:00000249 )

X scale

0.64 micrometer

Y scale

0.64 micrometer

Z scale

1.25 micrometer

T scale

5 minutes

Image Acquisition

Experiment type

Microscope type

Acquisition mode

Contrast method

Microscope model

Detector model

Objective model

Filter set

Summary of Methods

Asai K, Zhou Y, Takenouchi O, Kitajima TS. Artificial kinetochore beads establish a biorientation-like state in the spindle. Scince . 2024 Sep 20;385(6715):1366-1375.