SSBD:database

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Detail of FigS3C_mitosis_5

Project

422-Asai-KinetochoreBeads

SSBD:Repository

ssbd-repos-000422

Title

Z-series and time-lapse images of chromosomes and NDC80-NUF2 beads in embryo during the first mitosis

Description

Z-series and time-lapse images of chromosomes and NDC80-NUF2 beads in embryo during the first mitosis. NDC80-NUF2 (NDC80-NUF2 heterodimer) beads were formed in the embryo as follows; The authors microinjected mRNAs encoding C-terminally green fluorescent protein–tagged NDC80 (NDC80-GFP) and NUF2, followed by anti-GFP–conjugated microbeads into the cytoplasm. The mRNA for H2B-mCherry was also injected to visualize the chromosomes.

Release, Updated

2025-11-28

License

CC BY 4.0

Kind

Image data

File Formats

lsm

Data size

10.5 GB

Organism

Mus musculus ( NCBI:txid10090 )

Strain(s)

B6D2F1

Cell Line

Datatype

Molecular Function (MF)

Biological Process (BP)

nuclear chromosome segregation , kinetochore assembly

Cellular Component (CC)

kinetochore , chromosome

Biological Imaging Method

confocal microscopy ( Fbbi:00000251 )
time lapse microscopy ( Fbbi:00000249 )

X scale

0.07 micrometer

Y scale

0.07 micrometer

Z scale

1.25 micrometer

T scale

3 minutes

Image Acquisition

Experiment type

Microscope type

Acquisition mode

Contrast method

Microscope model

Detector model

Objective model

Filter set

Summary of Methods

Asai K, Zhou Y, Takenouchi O, Kitajima TS. Artificial kinetochore beads establish a biorientation-like state in the spindle. Scince . 2024 Sep 20;385(6715):1366-1375.