SSBD:database

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Detail of Fig2A_prometaphase_1_lsm

Project

422-Asai-KinetochoreBeads

SSBD:Repository

ssbd-repos-000422

Title

Z-series images of immunocytochemistry stained for NDC80-NUF2 beads, HURP, and DNA during prometaphase

Description

Z-series images of immunocytochemistry stained for NDC80-NUF2 beads, HURP (hepatoma-upregulated protein, a kinetochore fiber marker), and DNA during prometaphase.NDC80-NUF2 (NDC80-NUF2 heterodimer) beads were formed in the oocyte as follows; The authors microinjected mRNAs encoding C-terminally green fluorescent protein–tagged NDC80 (NDC80-GFP) and NUF2, followed by anti-GFP–conjugated microbeads into the cytoplasm. This .lsm dataset is the processed by Airyscan in the microscope system. The original data is the dataset; Fig2A_prometaphase_1_czi.

Release, Updated

2025-11-28

License

CC BY 4.0

Kind

Image data

File Formats

.lsm

Data size

821.6 MB

Organism

Mus musculus ( NCBI:txid10090 )

Strain(s)

B6D2F1

Cell Line

Datatype

Molecular Function (MF)

Biological Process (BP)

female meiosis chromosome segregation , kinetochore assembly

Cellular Component (CC)

kinetochore , chromosome

Biological Imaging Method

confocal microscopy ( Fbbi:00000251 )

X scale

0.03 micrometer

Y scale

0.03 micrometer

Z scale

0.101 micrometer

T scale

Image Acquisition

Experiment type

Microscope type

Acquisition mode

Contrast method

Microscope model

Detector model

Objective model

Filter set

Summary of Methods

Asai K, Zhou Y, Takenouchi O, Kitajima TS. Artificial kinetochore beads establish a biorientation-like state in the spindle. Scince . 2024 Sep 20;385(6715):1366-1375.