Summary of 279-Suzuki-CaspaseActivity

SSBD:database
SSBD:database URL
Title
Live imaging of caspase cascades activity in apoptosis-induced HeLa cells.
Description
-
Relase date
2023-07-20
Updated date
-
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
14 ( Image datasets: 14, Quantitative data datasets: 0 )
Size of Datasets
5.6 GB ( Image datasets: 5.6 GB, Quantitative data datasets: 0 bytes )

Organism(s)
Homo sapiens
Cell lines(s)
HeLa cell
Gene symbol(s)
NA
Protein name(s)
NA

Datatype
-
Molecular Function (MF)
cysteine-type endopeptidase activity involved in apoptotic process, cysteine-type endopeptidase activity
Biological Process (BP)
execution phase of apoptosis
Cellular Component (CC)
caspase complex
Biological Imaging Method
fluorescence microscopy, confocal microscopy
T scale
20 seconds of time interval

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

Miho Suzuki, Yutaka Shindo, Ryu Yamanaka, Kotaro Oka (2022) Live imaging of apoptotic signaling flow using tunable combinatorial FRET-based bioprobes for cell population analysis of caspase cascades., Scientific reports, Volume 12, Number 1, pp. 21160

Published in 2022 Dec 7 (Electronic publication in Dec. 7, 2022, midnight )

(Abstract) Understanding cellular signaling flow is required to comprehend living organisms. Various live cell imaging tools have been developed but challenges remain due to complex cross-talk between pathways and response heterogeneities among cells. We have focused on multiplex live cell imaging for statistical analysis to address the difficulties and developed simple multiple fluorescence imaging system to quantify cell signaling at single-cell resolution using Forster Resonance Energy Transfer (FRET)-based chimeric molecular sensors comprised of fluorescent proteins and dyes. The dye-fluorescent protein conjugate is robust for a wide selection of combinations, facilitating rearrangement for coordinating emission profile of molecular sensors to adjust for visualization conditions, target phenomena, and simultaneous use. As the molecular sensor could exhibit highly sensitive in detection for protease activity, we customized molecular sensor of caspase-9 and combine the established sensor for caspase-3 to validate the system by observation of caspase-9 and -3 dynamics simultaneously, key signaling flow of apoptosis. We found cumulative caspase-9 activity rather than reaction rate inversely regulated caspase-3 execution times for apoptotic cell death. Imaging-derived statistics were thus applied to discern the dominating aspects of apoptotic signaling unavailable by common live cell imaging and proteomics protein analysis. Adopted to various visualization targets, the technique can discriminate between rivalling explanations and should help unravel other protease involved signaling pathways.

Contact
Miho Suzuki , Graduate School of Science and Engineering, Saitama University , Department of Applied Chemistry
Contributors


Dataset List of 279-Suzuki-CaspaseActivity

#
Dataset ID
Kind
Size
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SSBD:OMERO
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# 10765
Dataset Kind Image data
Dataset Size 275.7 MB
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SSBD:OMERO
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# 10766
Dataset Kind Image data
Dataset Size 278.3 MB
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SSBD:OMERO
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# 10767
Dataset Kind Image data
Dataset Size 188.5 MB
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SSBD:OMERO
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# 10768
Dataset Kind Image data
Dataset Size 371.6 MB
4D view
SSBD:OMERO
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# 10769
Dataset Kind Image data
Dataset Size 371.6 MB
4D view
SSBD:OMERO
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# 10770
Dataset Kind Image data
Dataset Size 490.9 MB
4D view
SSBD:OMERO
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# 10771
Dataset Kind Image data
Dataset Size 521.7 MB
4D view
SSBD:OMERO
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# 10772
Dataset Kind Image data
Dataset Size 696.0 MB
4D view
SSBD:OMERO
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# 10773
Dataset Kind Image data
Dataset Size 506.3 MB
4D view
SSBD:OMERO
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# 10774
Dataset Kind Image data
Dataset Size 419.2 MB
4D view
SSBD:OMERO
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# 10775
Dataset Kind Image data
Dataset Size 467.9 MB
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SSBD:OMERO
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# 10776
Dataset Kind Image data
Dataset Size 314.1 MB
4D view
SSBD:OMERO
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# 10777
Dataset Kind Image data
Dataset Size 432.1 MB
4D view
SSBD:OMERO
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# 10778
Dataset Kind Image data
Dataset Size 432.1 MB
4D view
SSBD:OMERO
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