Detail of Figure3_Casp9_Casp3_0418-2


Project
SSBD:Repository
Title
Time-course live imaging of Caspase-9 and Caspase-3 activation in apoptotic HeLa cells.
Description
Time-course live imaging of Caspase-9 and Caspase-3 activation in apoptotic HeLa cells. You can refer to the URL to get the corresponding quantitative information about the fluorescence of the leakage ratios for each chromophore-derived fluorescence into each measurement channel while re-analysis is needed: https://ssbd.riken.jp/data/279-Suzuki-CaspaseActivity/supplement/Figure3(Dual)-Signal-Separation-Matrix.xls
Release, Updated
2023-07-20
License
CC BY
Kind
Image data
File Formats
.oib
Data size
521.7 MB

Organism
Homo sapiens ( NCBI:txid9606 )
Strain(s)
-
Cell Line
HeLa cell ( CLO_0003684 )

Datatype
-
Molecular Function (MF)
cysteine-type endopeptidase activity ( GO:0004197 ) , cysteine-type endopeptidase activity involved in apoptotic process ( GO:0097153 )
Biological Process (BP)
execution phase of apoptosis ( GO:0097194 )
Cellular Component (CC)
caspase complex ( GO:0008303 )
Biological Imaging Method
confocal microscopy ( Fbbi:00000251 )
fluorescence microscopy ( Fbbi:00000246 )
X scale
0.621 micrometer/pixel
Y scale
0.621 micrometer/pixel
Z scale
-
T scale
20 seconds of time interval

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
See details in Suzuki M, et. al. Sci Rep. 2022 Dec 7;12(1):21160.
Related paper(s)

Contact
Miho Suzuki , Graduate School of Science and Engineering, Saitama University , Department of Applied Chemistry
Contributors

OMERO Dataset
OMERO Project
Source