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Key
Organism
Strain
Cell Line
Gene Symbol
Protein name
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Gene Ontology
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Detail of Figure3_Casp9_Casp3_0703-2
SSBD:OMERO.gallery Dataset
SSBD:OMERO Dataset
SSBD:OMERO.gallery Project
SSBD:OMERO Project
4D View for Quantitative Data
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Project
279-Suzuki-CaspaseActivity
SSBD:Repository
ssbd-repos-000279
Title
Time-course live imaging of Caspase-9 and Caspase-3 activation in apoptotic HeLa cells.
Description
Time-course live imaging of Caspase-9 and Caspase-3 activation in apoptotic HeLa cells. You can refer to the URL to get the corresponding quantitative information about the fluorescence of the leakage ratios for each chromophore-derived fluorescence into each measurement channel while re-analysis is needed:
https://ssbd.riken.jp/data/279-Suzuki-CaspaseActivity/supplement/Figure3(Dual)-Signal-Separation-Matrix.xls
Release, Updated
2023-07-20
License
CC BY
Kind
Image data
File Formats
.oib
Data size
314.1 MB
Organism
Homo sapiens
(
NCBI:txid9606
)
Strain(s)
-
Cell Line
HeLa cell (
CLO_0003684
)
Datatype
-
Molecular Function (MF)
cysteine-type endopeptidase activity (
GO:0004197
) , cysteine-type endopeptidase activity involved in apoptotic process (
GO:0097153
)
Biological Process (BP)
execution phase of apoptosis (
GO:0097194
)
Cellular Component (CC)
caspase complex (
GO:0008303
)
Biological Imaging Method
confocal microscopy (
Fbbi:00000251
)
fluorescence microscopy (
Fbbi:00000246
)
X scale
0.621 micrometer/pixel
Y scale
0.621 micrometer/pixel
Z scale
-
T scale
20 seconds of time interval
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
See details in Suzuki M, et. al. Sci Rep. 2022 Dec 7;12(1):21160.
Related paper(s)
Contact
Miho Suzuki , Graduate School of Science and Engineering, Saitama University , Department of Applied Chemistry
Contributors
OMERO Dataset
https://ssbd.riken.jp/omero/webclient/?show=dataset-12424
OMERO Project
https://ssbd.riken.jp/omero/webclient/?show=project-1057
Source
https://ssbd.riken.jp/data/279-Suzuki-CaspaseActivity/source/Figure3_Casp9_Casp3_0703-2.zip
(169.8 MB)