394-Fukute-DNAunderwind
Author reviewedMechanical regulation of DNA underwinding in the nucleus
Project ID
394-Fukute-DNAunderwind
Project SID
394
Title
Mechanical regulation of DNA underwinding in the nucleus
Description
This project investigates how DNA is underwound in the mechanical environment of the nucleus, by performing super-resolution imaging of underwound DNA and nuclear proteins in mouse cells.
Submission Date
2024-09-26
Opened Date
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Release Date
2025-01-31
Update Date
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Kind
Image datasets
License
Project URL
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Project DOI
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Metadata Version
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Template Version
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Funding Information
This work was supported by JST, CREST, Grant Number JPMJCR2023, Japan (J.F., K.M.), JST, CREST, Grant Number JPMJCR22L5, Japan (T.A.), JST, SPRING, Grant Number JPMJSP2110, Japan (J.F.), MEXT/JSPS, KAKENHI, Grant Number JP23KJ1255, Japan (J.F.), and MEXT/JSPS, KAKENHI, Grant Number JP20K20180 and JP23K17196, Japan (K.M.).
Biosample
Organism
Mus musculus
(
NCBITaxon:10090
)
Strain
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Cell
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Cell line
MC3T3-E1 cell
(
CLO:0007582
)
MeSH
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Ontology
Anatomical Entity
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UBERON
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Biological Process
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Cellular Component
nucleus
(
GO:0005634
)
Molecular Function
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Medical Subject Headings
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Imaging Method
Method involved in biological imaging
Lattice-pattern structured illumination microscopy
Paper DOI / Paper URL
People
Contact
Koichiro Maki
Kyoto University
Laboratory of Cellular and Molecular Biomechanics, Department of Mammalian Regulatory Network, Graduate School of Biostudies
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Imaging dataset contributor
Jumpei Fukute (Kyoto University)
Koichiro Maki (Kyoto University)
Taiji Adachi (Kyoto University)
Quantitative dataset contributor
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Dataset List
Thumbnail
Dataset
Kind
Quantitative
Title
Image data
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Z-series Lattice-SIM images of nucleophosmin, DAPI and bio-psoralen in nucleus
Image data
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Z-series Lattice-SIM images of DAPI, bio-psoralen and RNAPIIS2P in nucleus
Image data
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Z-series Lattice-SIM images of DAPI, bio-psoralen and TOPI in a nucleus
Image data
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Lattice-SIM images of ribosome DNA, bio-psoralen, fibrillarin, and DAPI in nucleus
Image data
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Z-series Lattice-SIM images of bio-psoralen and fibrillarin in a nucleus
Image data
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Z-series Lattice-SIM images of bio-psoralen and nucleophosmin in nucleus
Image data
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Z-series Lattice-SIM images of bio-psoralen and RNA polymerase I in nucleus
Image data
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Z-series Lattice-SIM images of bio-psoralen and nucleophosmin in the presence of Actinomycin D
Image data
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Z-series Lattice-SIM images of bio-psoralen and nucleophosmin in the presence of CX-5461
Image data
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Z-series Lattice-SIM images of bio-psoralen and nucleophosmin in the absence of inhibitors
Image data
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Z-series Lattice-SIM images of bio-psoralen and nucleophosmin in the presence of TMPyP4
Image data
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Z-series Lattice-SIM images of bio-psoralen and nucleophosmin in the absence of TMPyP4
Image data
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Z-series Lattice-SIM images of bio-psoralen, fibrillarin and ribosome DNA in the presence of TMPyP4
Image data
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Lattice-SIM images of bio-psoralen, fibrillarin and ribosome DNA in the absence of TMPyP4