394-Fukute-DNAunderwind/Fig3a_DNAunderwind
Author reviewedLattice-SIM images of ribosome DNA, bio-psoralen, fibrillarin, and DAPI in nucleus
Project
Dataset ID
Fig3a_DNAunderwind
Kind
Image data
Title
Lattice-SIM images of ribosome DNA, bio-psoralen, fibrillarin, and DAPI in nucleus
Description
Lattice-SIM images of ribosome DNA, fibrillarin, DAPI and bio-psoralen in nucleus. The underwound DNA was labeled with biotinylated psoralen, which preferentially binds to underwound DNA rather than relaxed DNA, before cell fixation. Channel1;ribosome DNA, Chennel2;psoralen, Channel3;fibrillarin (a marker for fibrillar component of the nucleoli), Channel4;DAPI
Folder Name
Fig3a_DNAunderwind
File Format
.czi
File Size
50MB
File Separation Method
-
Figure Number
Fig3a
Dimensions
2560x2560x1x4x1
X Scale
0.05 micrometer
Y Scale
0.05 micrometer
Z Scale
-
T Scale
-
Channel
4 channel
Quantitative Data
-
Biosample
Description
MC3T3-E1 mouse osteoblast-like cells in which underwound nuclear DNA was fluorescently labeled with psoralen
Organism
Mus musculus (NCBITaxon:10090)
Strain
-
Cell
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Cell line
MC3T3-E1 cell (CLO:0007582)
MeSH
-
Intrinsic Variable
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Extrinsic Variable
The biotinylated psoralen was applied to the cell and crosslinked to DNA by UV irradiation. After cell fixation, NeutrAvidin Protein, DyLight^{TM} 488 was applied and other staining was conducted. The ribosome DNA was labeled with Cy3- labeld probes for mouse ribosome DNA (BAC clone RP23- 225M6, Chromosome Science Laboratory).
Ontology
Anatomical Entity
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UBERON
-
Biological Process
-
Cellular Component
nucleus (GO:0005634)
Molecular Function
-
MeSH Anatomy
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MeSH BP
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MeSH CC
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MeSH MF
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Genetics
Gene
-
Protein / Probe
-biotinylated psoralen, which preferentially binds to underwound DNA rather than relaxed DNA
Method / Genotype
DNA Binding
Gene
-
Protein / Probe
-Cy3
Method / Genotype
probe for ribosome DNA
Gene
Fbl
Protein / Probe
rRNA 2'-O-methyltransferase fibrillarin / Alexa Fluor 568
Method / Genotype
antibody
Gene
-
Protein / Probe
-DAPI
Method / Genotype
-
Treatments
No treatment records.
Imaging Method
Imaging Method
Lattice-pattern structured illumination microscopy
Detection Method
scientific complementary metal oxide semiconductor (sCMOS)
Visualization Method
Cy3,
NeutrAvidin Protein (DyLight^{TM} 488),
Alexa Fluor 568,
DAPI
Illumination Method
Lattice-pattern structured illumination
Source of Contrast
-
Imaged Parameter
fluorescence emission
Sample Preparation Method
formaldehyde fixed tissue
MeSH
-
Instrument
Body
Elyra7 super-resolution microscope (Zeiss)
Module
-
Light Source
-
Detector
-
Objective
Plan-Apochromat 40×/1.4 oil objective lens
Filter Set
-
Dichroic
-