394-Fukute-DNAunderwind/Fig2c_DNAunderwind
Author reviewedZ-series Lattice-SIM images of DAPI, bio-psoralen and TOPI in a nucleus
Project
Dataset ID
Fig2c_DNAunderwind
Kind
Image data
Title
Z-series Lattice-SIM images of DAPI, bio-psoralen and TOPI in a nucleus
Description
Z-series Lattice-SIM images of DAPI, bio-psoralen and TOPI in a nucleus. The underwound DNA was labeled with biotinylated psoralen, which preferentially binds to underwound DNA rather than relaxed DNA, before cell fixation. Channel1;TOPI (topoisomerase I (TopI), which is involved in the relaxation of under/over-wound DNA) , Chennel2;DAPI, Channel3;psoralen
Folder Name
Fig2c_DNAunderwind
File Format
.czi
File Size
60MB
File Separation Method
-
Figure Number
Fig2c
Dimensions
512x512x40x3x1
X Scale
0.05 micrometer
Y Scale
0.05 micrometer
Z Scale
0.44 micrometer
T Scale
-
Channel
3 channel
Quantitative Data
-
Biosample
Description
MC3T3-E1 mouse osteoblast-like cells in which underwound nuclear DNA was fluorescently labeled with psoralen
Organism
Mus musculus (NCBITaxon:10090)
Strain
-
Cell
-
Cell line
MC3T3-E1 cell (CLO:0007582)
MeSH
-
Intrinsic Variable
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Extrinsic Variable
The biotinylated psoralen was applied to the cell and crosslinked to DNA by UV irradiation. After cell fixation, NeutrAvidin Protein, DyLight^{TM} 488 was applied and other staining was conducted.
Ontology
Anatomical Entity
-
UBERON
-
Biological Process
-
Cellular Component
nucleus (GO:0005634)
Molecular Function
-
MeSH Anatomy
-
MeSH BP
-
MeSH CC
-
MeSH MF
-
Genetics
Gene
-
Protein / Probe
-biotinylated psoralen, which preferentially binds to underwound DNA rather than relaxed DNA
Method / Genotype
DNA Binding
Gene
Top1
Protein / Probe
DNA topoisomerase 1 / Alexa Fluor 568
Method / Genotype
antibody
Gene
-
Protein / Probe
-DAPI
Method / Genotype
-
Treatments
No treatment records.
Imaging Method
Imaging Method
Lattice-pattern structured illumination microscopy
Detection Method
scientific complementary metal oxide semiconductor (sCMOS)
Visualization Method
Alexa Fluor 568,
DAPI,
NeutrAvidin Protein (DyLight^{TM} 488)
Illumination Method
Lattice-pattern structured illumination
Source of Contrast
-
Imaged Parameter
fluorescence emission
Sample Preparation Method
formaldehyde fixed tissue
MeSH
-
Instrument
Body
Elyra7 super-resolution microscope (Zeiss)
Module
-
Light Source
-
Detector
-
Objective
Plan-Apochromat 40×/1.4 oil objective lens
Filter Set
-
Dichroic
-