394-Fukute-DNAunderwind/Fig4a_DNAunderwind_CX
Author reviewedZ-series Lattice-SIM images of bio-psoralen and nucleophosmin in the presence of CX-5461
Project
Dataset ID
Fig4a_DNAunderwind_CX
Kind
Image data
Title
Z-series Lattice-SIM images of bio-psoralen and nucleophosmin in the presence of CX-5461
Description
Z-series Lattice-SIM images of bio-psoralen, which labeled underwound DNA, DAPI and nucleophosmin in the presence of CX-5461. CX-5461 is an inhibitor of RNA polymerase I transcription initiation. Channel1;nucleophosmin (a marker for granular component forming the shell of the nucleoli), Chennel2;psoralen, Channel3;DAPI
Folder Name
Fig4a_DNAunderwind_CX
File Format
.czi
File Size
624MB
File Separation Method
-
Figure Number
Fig4a
Dimensions
2048x2048x26x3x1
X Scale
0.03 micrometer
Y Scale
0.03 micrometer
Z Scale
0.40 micrometer
T Scale
-
Channel
3 channel
Quantitative Data
-
Paper(s)
Biosample
Description
MC3T3-E1 mouse osteoblast-like cells in which underwound nuclear DNA was fluorescently labeled with psoralen
Organism
Mus musculus (NCBITaxon:10090)
Strain
-
Cell
-
Cell line
MC3T3-E1 cell (CLO:0007582)
MeSH
-
Intrinsic Variable
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Extrinsic Variable
To inhibit RNAPI transcription initiation, cells were treated with 3 micromolar CX-5461 for 15 min. The biotinylated psoralen was applied to the cell and crosslinked to DNA by UV irradiation. After cell fixation, NeutrAvidin Protein, DyLight^{TM} 488 was applied and other staining was conducted.
Ontology
Anatomical Entity
-
UBERON
-
Biological Process
-
Cellular Component
nucleus (GO:0005634)
Molecular Function
-
MeSH Anatomy
-
MeSH BP
-
MeSH CC
-
MeSH MF
-
Genetics
Gene
-
Protein / Probe
-biotinylated psoralen, which preferentially binds to underwound DNA rather than relaxed DNA
Method / Genotype
DNA Binding
Gene
Npm1
Protein / Probe
Nucleophosmin / Alexa Fluor 568
Method / Genotype
antibody
Gene
-
Protein / Probe
-DAPI
Method / Genotype
-
Treatments
Reagent / Compound
CX-5461
Concentration
3 micromolar
Fold Dilution
-
Imaging Method
Imaging Method
Lattice-pattern structured illumination microscopy
Detection Method
scientific complementary metal oxide semiconductor (sCMOS)
Visualization Method
Alexa Fluor 568,
DAPI,
NeutrAvidin Protein (DyLight^{TM} 488)
Illumination Method
Lattice-pattern structured illumination
Source of Contrast
-
Imaged Parameter
fluorescence emission
Sample Preparation Method
formaldehyde fixed tissue
MeSH
-
Instrument
Body
Elyra7 super-resolution microscope (Zeiss)
Module
-
Light Source
-
Detector
-
Objective
Plan-Apochromat 40×/1.4 oil objective lens
Filter Set
-
Dichroic
-