535-Ohashi-AxolotlSkinDCF
Author reviewedZ-stack images showing keratinocyte-driven dermal collagen formation in the axolotl skin
Project ID
535-Ohashi-AxolotlSkinDCF
Project SID
535
Title
Z-stack images showing keratinocyte-driven dermal collagen formation in the axolotl skin
Description
Type I collagen is a major component of the dermis and is formed by dermal fibroblasts. The development of dermal collagen structures has not been fully elucidated despite the major presence and importance of the dermis. This lack of understanding is due in part to the opacity of mammalian skin and it has been an obstacle to cosmetic and medical developments. We reveal the process of dermal collagen formation using the highly transparent skin of the axolotl and fluorescent collagen probes. We clarify that epidermal cells, not dermal fibroblasts, contribute to dermal collagen formation. Mesenchymal cells (fibroblasts) play a role in modifying the collagen fibers already built by keratinocytes. We confirm that collagen production by keratinocytes is a widely conserved mechanism in other model organisms. Our findings warrant a change in the current consensus about dermal collagen formation and could lead to innovations in cosmetology and skin medication.
Submission Date
2026-08-24
Opened Date
-
Release Date
2026-10-01
Update Date
-
Kind
Image datasets
License
Project URL
-
Project DOI
-
Metadata Version
-
Template Version
3.0.3
Funding Information
This work was supported by the following funding sources: JSPS Kakenhi (A.S.: #20H03264, #24K02034, #23H04314, and #24H01953), Koyanagi Foundation (A.S.), JST SPRING (#JPMJSP2126 A.O.), and the National Institute for Basic Biology Collaborative Research Programs, Trans-Scale Biology (#22NIBB101 to A.S.), and Integrative Imaging (22NIBB506 to A.S.).
Total
14 files / 2.4 GB
Image datasets
zipped 7 files / 991.4 MB, raw image 7 files / 1.4 GB, total 14 files / 2.4 GB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes
Biosample
Organism
Ambystoma mexicanum
(
NCBITaxon:8296
)
Strain
-
Cell
mesenchymal cell
(
CL:0008019
)
Cell line
-
MeSH
Ontology
Anatomical Entity
-
UBERON
Biological Process
extracellular matrix organization
(
GO:0030198
)
collagen metabolic process
(
GO:0032963
)
collagen fibril organization
(
GO:0030199
)
mesenchymal cell migration
(
GO:0090497
)
collagen biosynthetic process
(
GO:0032964
)
Cellular Component
extracellular matrix
(
GO:0031012
)
cytoplasm
(
GO:0005737
)
fibrillar collagen complex
(
GO:0098643
)
Molecular Function
-
Medical Subject Headings
Imaging Method
Method involved in biological imaging
confocal microscopy
(
FBbi:00000251
)
Paper(s)
People
Contact
Akira Satoh
Okayama University
Faculty of Environmental, Life, Natural Science and Technology
Laboratory for Organ Regeneration
Imaging dataset contributor
Ayaka Ohashi (Okayama University)
Quantitative dataset contributor
-
Image datasets
7
Quantitative datasets
0
Dataset List
Thumbnail
Dataset
Organism
Kind / Links
Z-stack images of type I collagen in the dorsal skin from a 12 centimeter long axolotl
Z-stack images of mesenchymal cells into the collagen layer in an 8 centimeter long axolotl
Z-stack images of the mature dermal collagen structure in a 12 centimeter long axolotl
Z-stack images of dermal collagen formation during growth from 5 to 8 centimeter body length in an axolotl
Z-stack images of dermal collagen formation during growth from 8 to 10 centimeter body length in an axolotl
Z-stack images of dermal collagen formation during growth from 10 to 12 centimeter body length in an axolotl
Z-stack images of dermal collagen formation during growth from 5 to 12 centimeter body length in an axolotl