Summary of 77-Aoki-CellDynERK
SSBD:database
SSBD:database URL
SSBD:repository
SSBD:repository URL
Title
Time-lapse imgaes and quantitative information of propagating wave of ERK activation orients collective cell migration
Description
-
Release date
2018-11-14
Updated date
2022-11-21
Errata
2022/11/21 Corrected the organism to Canis lupus familiaris/MDCK cell from Mus musculus by author's request.
License
CC BY
Kind
Image data, Quantitative data
based on Experiment
Number of Datasets
16
( Image datasets: 14,
Quantitative data datasets: 2 )
Size of Datasets
1.4 GB
( Image datasets: 1.4 GB,
Quantitative data datasets: 548.1 KB )
Organism(s)
Canis lupus familiaris
Cell lines(s)
MDCK cell
Protein name(s)
CRaf, ERK, H2B, KRasCT, MCL, MEK
Protein tag(s)
mCherry
Datatype
cell dynamics
Molecular Function (MF)
Biological Process (BP)
activation of MAP ERK kinase kinase
Cellular Component (CC)
-
Biological Imaging Method
-
XYZ Scale
XY: 0.91 micrometer/pixel, Z: NA, XY: 1.48 micrometer/pixel, Z: NA, XY: 0.65 micrometer/pixel, Z: NA, XY: 0.49 micrometer/pixel, Z: NA , XY: 1.48 micrometer/pixel, Z: NA , XY: 0.65 micrometer/pixel, Z: NA , XY: 0.49 micrometer/pixel, Z: NA
T scale
180 second for each time interval, 120 second for each time interval
Image Acquisition
Experiment type
, TimeLapse
Microscope type
, FluorescenceMicroscope
Acquisition mode
BrightField,
Contrast method
, Fluorescence
Microscope model
Olympus IX81,
Detector model
, Photometrics,Tucson,AZ
Objective model
, UPlanSApo 20x/0.75 or UJPlanSApo 60x/1.45, UPlanSApo 20x/0.75 or UJPlanSApo 60x/1.43, UPlanSApo 20x/0.75 or UJPlanSApo 60x/1.48, UPlanSApo 20x/0.75 or UJPlanSApo 60x/1.35, UPlanSApo 20x/0.75 or UJPlanSApo 60x/1.36, UPlanSApo 20x/0.75 or UJPlanSApo 60x/1.40, UPlanSApo 20x/0.75 or UJPlanSApo 60x/1.44, UPlanSApo 20x/0.75 or UJPlanSApo 60x/1.39, UPlanSApo 20x/0.75 or UJPlanSApo 60x/1.47, UPlanSApo 20x/0.75 or UJPlanSApo 60x/1.42, UPlanSApo 20x/0.75 or UJPlanSApo 60x/1.41, UPlanSApo 20x/0.75 or UJPlanSApo 60x/1.46, UPlanSApo 20x/0.75 or UJPlanSApo 60x/1.37, UPlanSApo 20x/0.75 or UJPlanSApo 60x/1.38
Filter set
FF01-438/24(Semrock, Rochester, NY);FF01-483/32(Semrock), , FF01-438/24(Semrock, Rochester, NY);FF01-542/27(Semrock), FF01-641/75(Semrock);FF01-692/LP(Semrock), FF01-580/20(Semrock);FF01-632/22(Semrock)
Related paper(s)
Contact
Kazuhiro Aoki
, National Institutes of Natural Sciences
, National Institute for Basic Biology
, Division of Quantitative Biology
Contributors
Kazuhiro Aoki, Yohei Kondo, Honda Naoki, Toru Hiratsuka, Reina E. Itoh, Michiyuki Matsuda
Dataset List of 77-Aoki-CellDynERK
Thumbnail
#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
Download BDML
Download Images
Thumbnail
#
4019
Datast ID
Figure1B
Dataset Kind
Image data
Dataset Size
784.9 MB
4D view
SSBD:OMERO
Download BDML
Download Image data
Thumbnail
#
4020
Datast ID
Figure1G
Dataset Kind
Image data
Dataset Size
49.3 MB
4D view
SSBD:OMERO
Download BDML
Download Image data
Thumbnail
#
4021
Datast ID
Figure4A
Dataset Kind
Image data
Dataset Size
241.2 MB
4D view
SSBD:OMERO
Download BDML
Download Image data
Thumbnail
#
4022
Datast ID
Figure4A_ERK
Dataset Kind
Image data
Dataset Size
3.6 MB
4D view
SSBD:OMERO
Download BDML
Download Image data
Thumbnail
#
4023
Datast ID
Figure4A_merge
Dataset Kind
Image data
Dataset Size
3.6 MB
4D view
SSBD:OMERO
Download BDML
Download Image data
Thumbnail
#
4024
Datast ID
Figure4A_MLC
Dataset Kind
Image data
Dataset Size
3.6 MB
4D view
SSBD:OMERO
Download BDML
Download Image data
Thumbnail
#
4025
Datast ID
Figure5A
Dataset Kind
Image data
Dataset Size
1.7 MB
4D view
SSBD:OMERO
Download BDML
Download Image data
Thumbnail
#
4026
Datast ID
Figure6B_iRFP
Dataset Kind
Image data
Dataset Size
24.8 MB
4D view
SSBD:OMERO
Download BDML
Download Image data
Thumbnail
#
4027
Datast ID
Figure6B_mCherry_ERK
Dataset Kind
Image data
Dataset Size
24.8 MB
4D view
SSBD:OMERO
Download BDML
Download Image data
Thumbnail
#
4028
Datast ID
Figure6C_opticflow
Dataset Kind
Image data
Dataset Size
7.4 MB
4D view
SSBD:OMERO
Download BDML
Download Image data
Thumbnail
#
4029
Datast ID
FigureS1A_ADAM10_inhibitor
Dataset Kind
Image data
Dataset Size
128.1 MB
4D view
SSBD:OMERO
Download BDML
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Thumbnail
#
4030
Datast ID
FigureS1A_DMSO
Dataset Kind
Image data
Dataset Size
128.1 MB
4D view
SSBD:OMERO
Download BDML
Download Image data
Thumbnail
#
4031
Datast ID
FigureS4_Beads
Dataset Kind
Image data
Dataset Size
1.3 MB
4D view
SSBD:OMERO
Download BDML
Download Image data
Thumbnail
#
4032
Datast ID
FigureS4_Ratio
Dataset Kind
Image data
Dataset Size
1.9 MB
4D view
SSBD:OMERO
Download BDML
Download Image data
Thumbnail
-
#
4033
Datast ID
Figure4C
Dataset Kind
Quantitative data
Dataset Size
548.1 KB
4D view
SSBD:OMERO
Download BDML
Download Image data