Detail of Figure6C_opticflow
Project
SSBD:Repository
Title
Montage images of the optic flow of cell migration in MDCK cells expresssing CRY2-CRaf, CIBN-EGFP-KRasCT, FLAG-MEK-mCFP, mCherry-ERK, and histone H2B-iRFP.
Description
NA
Release, Updated
2018-11-14,
2022-11-21
2022-11-21
Errata in Project
2022/11/21 Corrected the organism to Canis lupus familiaris/MDCK cell from Mus musculus by author's request.
License
CC BY
Kind
Image data
based on Experiment
File Formats
Data size
7.4 MB
Protein names
CRaf, KRasCT, MEK, ERK, H2B
Datatype
cell dynamics
Molecular Function (MF)
Biological Process (BP)
activation of MAP ERK kinase kinase
(
GO:0000186
)
Cellular Component (CC)
-
Biological Imaging Method
XYZ Scale
XY: 1.48 micrometer/pixel, Z: NA
T scale
120 second for each time interval
Image Acquisition
Experiment type
TimeLapse
Microscope type
FluorescenceMicroscope
Acquisition mode
BrightField
Contrast method
Fluorescence
Microscope model
Olympus IX81
Detector model
Photometrics,Tucson,AZ
Objective model
UPlanSApo 20x/0.75 or UJPlanSApo 60x/1.44
Filter set
Summary of Methods
See details in Aoki et al. (2017) Dev Cell, 43(3): 305-317.
Related paper(s)
Contact
Kazuhiro Aoki
, National Institutes of Natural Sciences
, National Institute for Basic Biology
, Division of Quantitative Biology
Contributors
Kazuhiro Aoki, Yohei Kondo, Honda Naoki, Toru Hiratsuka, Reina E. Itoh, Michiyuki Matsuda
OMERO Dataset
OMERO Project