Detail of Figure6C_opticflow
Project
Title
Montage images of the optic flow of cell migration in MDCK cells expresssing CRY2-CRaf, CIBN-EGFP-KRasCT, FLAG-MEK-mCFP, mCherry-ERK, and histone H2B-iRFP.
Description
NA
Release, Updated
2018-11-14,
2022-11-21
2022-11-21
Errata in Project
2022/11/21 Corrected the organism to Canis lupus familiaris/MDCK cell from Mus musculus by author's request.
License
CC BY
Kind
Image data
based on Experiment
File Formats
Data size
7.4 MB
Protein names
CRaf, KRasCT, MEK, ERK, H2B
Datatype
cell dynamics
Molecular Function (MF)
Biological Process (BP)
activation of MAP ERK kinase kinase
(
GO:0000186
)
Cellular Component (CC)
-
Biological Imaging Method
XYZ Scale
XY: 1.48 micrometer/pixel, Z: NA
T scale
120 second for each time interval
Image Acquisition
Experiment type
TimeLapse
Microscope type
FluorescenceMicroscope
Acquisition mode
BrightField
Contrast method
Fluorescence
Microscope model
Olympus IX81
Detector model
Photometrics,Tucson,AZ
Objective model
UPlanSApo 20x/0.75 or UJPlanSApo 60x/1.44
Filter set
Summary of Methods
See details in Aoki et al. (2017) Dev Cell, 43(3): 305-317.
Related paper(s)
Kazuhiro Aoki, Yohei Kondo, Honda Naoki, Toru Hiratsuka, Reina E Itoh, Michiyuki Matsuda (2017) Propagating Wave of ERK Activation Orients Collective Cell Migration., Developmental cell, Volume 43, Number 3, pp. 305-317.e5
Published in 2017 Nov 6
- doi: 10.1016/j.devcel.2017.10.016
-
pmid: 29112851
(Abstract) The biophysical framework of collective cell migration has been extensively investigated in recent years; however, it remains elusive how chemical inputs from neighboring cells are integrated to coordinate the collective movement. Here, we provide evidence that propagation waves of extracellular signal-related kinase (ERK) mitogen-activated protein kinase activation determine the direction of the collective cell migration. A wound-healing assay of Mardin-Darby canine kidney (MDCK) epithelial cells revealed two distinct types of ERK activation wave, a "tidal wave" from the wound, and a self-organized "spontaneous wave" in regions distant from the wound. In both cases, MDCK cells collectively migrated against the direction of the ERK activation wave. The inhibition of ERK activation propagation suppressed collective cell migration. An ERK activation wave spatiotemporally controlled actomyosin contraction and cell density. Furthermore, an optogenetic ERK activation wave reproduced the collective cell migration. These data provide new mechanistic insight into how cells sense the direction of collective cell migration.(MeSH Terms)
Contact
Kazuhiro Aoki
, National Institutes of Natural Sciences
, National Institute for Basic Biology
, Division of Quantitative Biology
Contributors
Kazuhiro Aoki, Yohei Kondo, Honda Naoki, Toru Hiratsuka, Reina E. Itoh, Michiyuki Matsuda
OMERO Dataset
OMERO Project
Source