SSBD:database

Key Organism Strain Cell Line Gene Symbol Protein name Protein tag Reporter Project ID Dataset ID Title Description Data Type Gene Ontology Biological Imaging Methods Method Summary Paper Information Medical Subject Headlines Person

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469-Ishii-pulseSTED

https://ssbd.riken.jp/database/469-Ishii-pulseSTED/

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ssbd-repos-000469

https://ssbd.riken.jp/repository/469/

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Super resolution image of dendritic spine taken by new STED microscopy

Super-resolution in two-photon excitation (2PE) microscopy offers new approaches for visualizing the deep inside the brain functions at the nanoscale. In this study, the authors developed a novel 2PE stimulated-emission-depletion (STED) microscope with all-synchronized picosecond pulse light sources and time-gated fluorescence detection, namely, all-pulsed 2PE-gSTED microscopy. The implementation of time-gating is critical to excluding undesirable signals derived from brain tissues. Even in a case using subnanosecond pulses for STED, the impact of time-gating was not negligible; the spatial resolution in the image of the brain tissue was improved by approximately 1.4 times compared with non time-gated image. This finding demonstrates that time-gating is more useful than previously thought for improving spatial resolution in brain tissue imaging. This microscopy will facilitate deeper super-resolution observation of the fine structure of neuronal dendritic spines and the intracellular dynamics in brain tissue.

2026-07-08

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CC BY 4.0

Image data based on Experiment

10 ( Image datasets: 10, Quantitative data datasets: 0 )

78.6 MB ( Image datasets: 78.6 MB, Quantitative data datasets: 0 bytes )

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Mus musculus

B6.Cg-Tg(Thy1-YFP)HJrs/J

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dendritic spine

two-photon laser scanning microscopy, stimulated emission depletion

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Hirokazu Ishii, Kohei Otomo, Ching-Pu Chang, Miwako Yamasaki, Masahiko Watanabe, Hiroyuki Yokoyama, Tomomi Nemoto (2023) All-synchronized picosecond pulses and time-gated detection improve the spatial resolution of two-photon STED microscopy in brain tissue imaging., PLOS ONE, Volume 18, Number 8

Published in 2023 (Electronic publication in Aug. 24, 2023, midnight )

• doi: 10.1371/journal.pone.0290550
• pmid: 37616194
  

(Abstract) Super-resolution in two-photon excitation (2PE) microscopy offers new approaches for visualizing the deep inside the brain functions at the nanoscale. In this study, we developed a novel 2PE stimulated-emission-depletion (STED) microscope with all-synchronized picosecond pulse light sources and time-gated fluorescence detection, namely, all-pulsed 2PE-gSTED microscopy. The implementation of time-gating is critical to excluding undesirable signals derived from brain tissues. Even in a case using subnanosecond pulses for STED, the impact of time-gating was not negligible; the spatial resolution in the image of the brain tissue was improved by approximately 1.4 times compared with non time-gated image. This finding demonstrates that time-gating is more useful than previously thought for improving spatial resolution in brain tissue imaging. This microscopy will facilitate deeper super-resolution observation of the fine structure of neuronal dendritic spines and the intracellular dynamics in brain tissue.

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Tomomi Nemoto , National Institutes of Natural Sciences , Exploratory Research Center on Life and Living Systems , Exploratory Research Center on Life and Living Systems

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