Detail of Fig4B_2PE-gSTED
Project
SSBD:Repository
Title
2PE-gSTED image of the neuronal dendrites in immunostained Thy1-YFP-H mouse brain
Description
2PE (Two-photon excitation microscopy)-gSTED image of the neuronal dendrites in immunostained Thy1-YFP-H mouse brain slice. The slice was immunostained with anti-GFP antibody, and visualized by AlexaFluor532-labeled secondary antibody. In this STED (stimulated-emission-depletion) imaging, the picoseconds excitation light pulse was applied and the detection was synchronized.
Release, Updated
2026-07-08
License
CC BY 4.0
Kind
Image data
File Formats
.tif
Data size
256.3 KB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
Cellular Component (CC)
dendritic spine
Biological Imaging Method
two-photon laser scanning microscopy
(
Fbbi:00000254
)
stimulated emission depletion ( Fbbi:00000334 )
stimulated emission depletion ( Fbbi:00000334 )
X scale
0.04 micrometer
Y scale
0.04 micrometer
Z scale
-
T scale
-
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Ishii H, Otomo K, Chang CP, Yamasaki M, Watanabe M, Yokoyama H, Nemoto T. All-synchronized picosecond pulses and time-gated detection improve the spatial resolution of two-photon STED microscopy in brain tissue imaging. PLoS One. 2023 Aug 24;18(8):e0290550.
Related paper(s)
Contact
Tomomi Nemoto
, National Institutes of Natural
Sciences
, Exploratory Research Center on Life and Living Systems
, Exploratory Research Center on Life and Living Systems
Contributors
OMERO Project