Summary of 199-Ichimura-MulticellularDyn

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SSBD:repository URL

Title
Imaging data of centimeter-scale dynamics of multicellular systems observed with sub-cellular resolution.
Description
-
Relase date
2022-11-23
Updated date
-
License
CC-BY-NC
Kind
Image data based on Experiment
Number of Datasets
7 ( Image datasets: 7, Quantitative data datasets: 0 )
Size of Datasets
527.2 GB ( Image datasets: 527.2 GB, Quantitative data datasets: 0 bytes )

Organism(s)
Homo sapiens, Mus musculus, Canis lupus familiaris, Dictyostelium discoideum
Cell lines(s)
MDCK cell, HeLa cell
Protein tag(s)
Flamindo2, RFP

Datatype
-
Molecular Function (MF)
Biological Process (BP)
cAMP-mediated signaling, calcium-mediated signaling
Cellular Component (CC)
nucleus
Biological Imaging Method
fluorescence microscopy, FRET
X scale
1.1 micrometer/pixel
Y scale
1.1 micrometer/pixel
Z scale
-
T scale
5 seconds per frame, NA, 9.4 frames per second, 30 seconds per frame

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

T Ichimura, T Kakizuka, K Horikawa, K Seiriki, A Kasai, H Hashimoto, K Fujita, T M Watanabe, T Nagai (2021) Exploring rare cellular activity in more than one million cells by a transscale scope., Scientific reports, Volume 11, Number 1, pp. 16539

Published in 2021 Aug 16 (Electronic publication in Aug. 16, 2021, midnight )

(Abstract) In many phenomena of biological systems, not a majority, but a minority of cells act on the entire multicellular system causing drastic changes in the system properties. To understand the mechanisms underlying such phenomena, it is essential to observe the spatiotemporal dynamics of a huge population of cells at sub-cellular resolution, which is difficult with conventional tools such as microscopy and flow cytometry. Here, we describe an imaging system named AMATERAS that enables optical imaging with an over-one-centimeter field-of-view and a-few-micrometer spatial resolution. This trans-scale-scope has a simple configuration, composed of a low-power lens for machine vision and a hundred-megapixel image sensor. We demonstrated its high cell-throughput, capable of simultaneously observing more than one million cells. We applied it to dynamic imaging of calcium ions in HeLa cells and cyclic-adenosine-monophosphate in Dictyostelium discoideum, and successfully detected less than 0.01% of rare cells and observed multicellular events induced by these cells.
(MeSH Terms)
Related paper(s)

Ichimura, T., Kakizuka, T., Horikawa, K., Seiriki, K., Kasai, A., Hashimoto, H., Fujita, K., Watanabe, T. M., Nagai, T. (2021/01/01), Exploring rare cellular activity in more than one million cells by a trans-scale-scope, 2020.06.29.179044

Published in 2021/01/01

(Abstract) In many phenomena of biological systems, not a majority, but a minority of cells act on the entire multicellular system causing drastic changes in the system properties. To understand the mechanisms underlying such phenomena, it is essential to observe the spatiotemporal dynamics of a huge population of cells at sub-cellular resolution, which is difficult with conventional tools such as microscopy and flow cytometry. Here, we describe an imaging system named AMATERAS that enables optical imaging with an over-one-centimeter field-of-view and a-few-micrometer spatial resolution. This trans-scale-scope has a simple configuration, composed of a low-power lens for machine vision and a hundred-megapixel image sensor. We demonstrated its high cell-throughput, capable of simultaneously observing more than one million cells. We applied it to dynamic imaging of calcium ions in HeLa cells and cyclic-adenosine-monophosphate in Dictyostelium discoideum, and successfully detected less than 0.01% of rare cells and observed multicellular events induced by these cells.Competing Interest StatementThe authors have declared no competing interest.

Contact
Takeharu Nagai , Osaka University, SANKEN (The Institute of Scientific and Industrial Research)
Contributors


Dataset List of 199-Ichimura-MulticellularDyn

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
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# 7724
Datast ID Fig2_BrainSliceRGB
Dataset Kind Image data
Dataset Size 348.4 MB
4D view
SSBD:OMERO
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# 7725
Datast ID Fig3_MDCKpostconfl
Dataset Kind Image data
Dataset Size 232.3 MB
4D view
SSBD:OMERO
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# 7726
Datast ID Fig3_MDCKpreconfl
Dataset Kind Image data
Dataset Size 232.3 MB
4D view
SSBD:OMERO
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# 7727
Dataset Kind Image data
Dataset Size 45.4 GB
4D view
SSBD:OMERO
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# 7728
Dataset Kind Image data
Dataset Size 45.4 GB
4D view
SSBD:OMERO
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# 7729
Datast ID Fig5-6_Flamindo2
Dataset Kind Image data
Dataset Size 217.8 GB
4D view
SSBD:OMERO
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# 7730
Datast ID Fig5-6_RFP
Dataset Kind Image data
Dataset Size 217.8 GB
4D view
SSBD:OMERO
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