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Detail of Fig3_MDCKpostconfl

T Ichimura, T Kakizuka, K Horikawa, K Seiriki, A Kasai, H Hashimoto, K Fujita, T M Watanabe, T Nagai (2021) Exploring rare cellular activity in more than one million cells by a transscale scope., Scientific reports, Volume 11, Number 1, pp. 16539

Published in 2021 Aug 16 (Electronic publication in Aug. 16, 2021, midnight )

• doi: 10.1038/s41598-021-95930-7
• pmid: 34400683
  
• url: https://www.nature.com/articles/s41598-021-95930-7
  

(Abstract) In many phenomena of biological systems, not a majority, but a minority of cells act on the entire multicellular system causing drastic changes in the system properties. To understand the mechanisms underlying such phenomena, it is essential to observe the spatiotemporal dynamics of a huge population of cells at sub-cellular resolution, which is difficult with conventional tools such as microscopy and flow cytometry. Here, we describe an imaging system named AMATERAS that enables optical imaging with an over-one-centimeter field-of-view and a-few-micrometer spatial resolution. This trans-scale-scope has a simple configuration, composed of a low-power lens for machine vision and a hundred-megapixel image sensor. We demonstrated its high cell-throughput, capable of simultaneously observing more than one million cells. We applied it to dynamic imaging of calcium ions in HeLa cells and cyclic-adenosine-monophosphate in Dictyostelium discoideum, and successfully detected less than 0.01% of rare cells and observed multicellular events induced by these cells.

• Animals
• Brain/cytology
• Calcium/analysis
• Cells/cytology[major]
• Cyclic AMP/analysis
• Dictyostelium/chemistry
• Dictyostelium/ultrastructure
• Dogs
• Entosis
• Epithelial Cells/ultrastructure
• Equipment Design
• Green Fluorescent Proteins
• HeLa Cells/chemistry
• HeLa Cells/ultrastructure
• Humans
• Interneurons/ultrastructure
• Luminescent Proteins
• Madin Darby Canine Kidney Cells
• Mice
• Microscopy, Fluorescence/instrumentation
• Microscopy, Fluorescence/methods[major]
• Neurons/ultrastructure
• Semiconductors

Ichimura, T., Kakizuka, T., Horikawa, K., Seiriki, K., Kasai, A., Hashimoto, H., Fujita, K., Watanabe, T. M., Nagai, T. (2021/01/01), Exploring rare cellular activity in more than one million cells by a trans-scale-scope, 2020.06.29.179044

Published in 2021/01/01

• doi: 10.1101/2020.06.29.179044

(Abstract) In many phenomena of biological systems, not a majority, but a minority of cells act on the entire multicellular system causing drastic changes in the system properties. To understand the mechanisms underlying such phenomena, it is essential to observe the spatiotemporal dynamics of a huge population of cells at sub-cellular resolution, which is difficult with conventional tools such as microscopy and flow cytometry. Here, we describe an imaging system named AMATERAS that enables optical imaging with an over-one-centimeter field-of-view and a-few-micrometer spatial resolution. This trans-scale-scope has a simple configuration, composed of a low-power lens for machine vision and a hundred-megapixel image sensor. We demonstrated its high cell-throughput, capable of simultaneously observing more than one million cells. We applied it to dynamic imaging of calcium ions in HeLa cells and cyclic-adenosine-monophosphate in Dictyostelium discoideum, and successfully detected less than 0.01% of rare cells and observed multicellular events induced by these cells.Competing Interest StatementThe authors have declared no competing interest.