SSBD:database

Jun Takayama, Shuichi Onami (2016) The Sperm TRP-3 Channel Mediates the Onset of a Ca(2+) Wave in the Fertilized C. elegans Oocyte., Cell reports, Volume 15, Number 3, pp. 625-637

Published in 2016 Apr 19 (Electronic publication in April 7, 2016, midnight )

• doi: 10.1016/j.celrep.2016.03.040
• pmid: 27068469
  

(Abstract) Sperm induce Ca(2+) waves in the fertilized egg by introducing soluble factors or by surface interactions, which activate egg Ca(2+) channels. Involvement of sperm Ca(2+) channels is predicted by the conduit model; however, this model has not been validated. In Caenorhabditis elegans, the sperm-specific TRP family Ca(2+) channel TRP-3 mediates sperm-oocyte fusion. Here, using high-speed in vivo imaging and image analyses, we show that sperm induce an immediate local Ca(2+) rise followed by a Ca(2+) wave in fertilized C. elegans oocytes. Oocytes fertilized by rare trp-3 escaper sperm showed a lack of local rise and a delay in onset of the Ca(2+) wave. Sperm Ca(2+) imaging suggests that the local rise is not due to the bolus introduction of stored Ca(2+). These results suggest that, along with its primary function in sperm-oocyte fusion, TRP-3 induces Ca(2+) waves in fertilized oocytes, consistent with the conduit model.

• Animals
• Animals, Genetically Modified
• Caenorhabditis elegans/cytology
• Caenorhabditis elegans/metabolism[major]
• Caenorhabditis elegans Proteins/metabolism[major]
• Calcium Channels/metabolism[major]
• Calcium Signaling[major]
• Fertilization[major]
• Gene Expression Regulation, Developmental
• Genetic Loci
• Homeostasis
• Male
• Membrane Fusion
• Models, Biological
• Oocytes/cytology
• Oocytes/metabolism[major]
• Spermatozoa/metabolism[major]
• Time Factors
• Transgenes