Detail of G2AN_TM9.5_L



Project
SSBD:Repository
Title
brain sections of a P7 mouse (ID:1) that was birthdate tagged at E9.5 (TM9.5) and visualized for nucleus-localized beta-galactosidase reporter
Description
DAB immunostaining with rabbit anti-beta-galactosidase antibody (A11132, Molecular Probes)
Release, Updated
2022-04-15
License
CC BY
Kind
Image data
File Formats
PNG
Data size
19.3 GB

Organism
Mus musculus ( NCBITaxon:10090 )
Strain(s)
C57BL/6 Neurog2CreER(G2A); TaumGFP-nLacZ
Cell Line
-
Reporter
DAB, anti-beta-galactosidase antibody

Datatype
-
Molecular Function (MF)
-
Biological Process (BP)
nervous system development ( GO:0007399 )
Cellular Component (CC)
nucleus ( GO:0005634 ) cell body ( GO:0044297 )
Biological Imaging Method
bright-field microscopy ( Fbbi:00000243 )
X scale
1.0 micrometer/pixel
Y scale
1.0 micrometer/pixel
Z scale
20 micrometer/slice
T scale
-

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
See details in Hirata et al., NeuroGT: A brain atlas of neurogenic tagging CreER drivers for birthdate-based classification and manipulation of mouse neurons, Cell Reports Methods 100012, 2021
Related paper(s)

Tatsumi Hirata, Yukako Tohsato, Hiroya Itoga, Go Shioi, Hiroshi Kiyonari, Sanae Oka, Toshihiko Fujimori, Shuichi Onami (2021) NeuroGT: A brain atlas of neurogenic tagging CreER drivers for birthdate-based classification and manipulation of mouse neurons., Cell reports methods, Volume 1, Number 3, pp. 100012

Published in 2021 Jul 26 (Electronic publication in May 25, 2021, midnight )

(Abstract) Neuronal birthdate is one of the major determinants of neuronal phenotypes. However, most birthdating methods are retrospective in nature, allowing very little experimental access to the classified neuronal subsets. Here, we introduce four neurogenic tagging mouse lines, which can assign CreER-loxP recombination to neuron subsets that share the same differentiation timing in living animals and enable various experimental manipulations of the classified subsets. We constructed a brain atlas of the neurogenic tagging mouse lines (NeuroGT), which includes holistic image data of the loxP-recombined neurons and their processes across the entire brain that were tagged on each single day during the neurodevelopmental period. This image database, which is open to the public, offers investigators the opportunity to find specific neurogenic tagging driver lines and the stages of tagging appropriate for their own research purposes.

Contact
Tatsumi Hirata , National Institute of Genetics , Brain Function Laboratory
Contributors
Toshihiko Fujimori

OMERO Dataset
OMERO Project
Source