Detail of D4AN_TM9.5_G


Project
SSBD:Repository
Title
brain sections of a P7 mouse (ID:1) that was birthdate tagged at E9.5 (TM9.5) and visualized for membrane-localized GFP reporter
Description
DAB immunostaining with rabbit anti-GFP antibody (#598, MBL)
Release, Updated
2022-04-15
License
CC BY
Kind
Image data
File Formats
PNG
Data size
24.4 GB

Organism
Mus musculus ( NCBI:txid10090 )
Strain(s)
C57BL/6 Neurod4CreER(D4A); TaumGFP-nLacZ
Cell Line
-
Reporter
DAB, anti-GFP antibody

Datatype
-
Molecular Function (MF)
-
Biological Process (BP)
nervous system development ( GO:0007399 )
Cellular Component (CC)
plasma membrane ( GO:0005886 ) , neuron projection ( GO:0043005 )
Biological Imaging Method
bright-field microscopy ( Fbbi:00000243 )
X scale
1.0 micrometer/pixel
Y scale
1.0 micrometer/pixel
Z scale
20 micrometer/slice
T scale
-

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
See details in Hirata et al., NeuroGT: A brain atlas of neurogenic tagging CreER drivers for birthdate-based classification and manipulation of mouse neurons, Cell Reports Methods 100012, 2021
Related paper(s)

Contact
Tatsumi Hirata , National Institute of Genetics , Brain Function Laboratory
Contributors
Toshihiko Fujimori

OMERO Dataset
OMERO Project
Source