Summary of 9-Masumoto-Transcriptome

SSBD:database
SSBD:database URL
Title
-
Description
-
Relase date
2014-10-03
Updated date
2017-10-03
License
CC BY-SA
Kind
Quantitative data based on Experiment
Number of Datasets
8 ( Image datasets: 0, Quantitative data datasets: 8 )
Size of Datasets
97.0 MB ( Image datasets: 0 bytes, Quantitative data datasets: 97.0 MB )

Organism(s)
M. musculus
Gene symbol(s)
NA
Protein name(s)
NA

Datatype
gene expression data
Molecular Function (MF)
Biological Process (BP)
-
Cellular Component (CC)
-
Biological Imaging Method
-
XYZ Scale
-
T scale
-

Image Acquisition
Experiment type
Other
Microscope type
Other
Acquisition mode
Other
Contrast method
Other
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

Koh-Hei Masumoto, Maki Ukai-Tadenuma, Takeya Kasukawa, Mamoru Nagano, Kenichiro D Uno, Kaori Tsujino, Kazumasa Horikawa, Yasufumi Shigeyoshi, Hiroki R Ueda (2010) Acute induction of Eya3 by late-night light stimulation triggers TSHbeta expression in photoperiodism., Current biology : CB, Volume 20, Number 24, pp. 2199-206

Published in 2010 Dec 21 (Electronic publication in Dec. 2, 2010, midnight )

(Abstract) Living organisms detect seasonal changes in day length (photoperiod) [1-3] and alter their physiological functions accordingly to fit seasonal environmental changes. TSHbeta, induced in the pars tuberalis (PT), plays a key role in the pathway that regulates vertebrate photoperiodism [4, 5]. However, the upstream inducers of TSHbeta expression remain unknown. Here we performed genome-wide expression analysis of the PT under chronic short-day and long-day conditions in melatonin-proficient CBA/N mice, in which the photoperiodic TSHbeta expression response is preserved [6]. This analysis identified "short-day" and "long-day" genes, including TSHbeta, and further predicted the acute induction of long-day genes by late-night light stimulation. We verified this by advancing and extending the light period by 8 hr, which induced TSHbeta expression within one day. In the following genome-wide expression analysis under this acute long-day condition, we searched for candidate upstream genes by looking for expression that preceded TSHbeta's, and we identified the Eya3 gene. We demonstrated that Eya3 and its partner Six1 synergistically activate TSHbeta expression and that this activation is further enhanced by Tef and Hlf. These results elucidate the comprehensive transcriptional photoperiodic response in the PT, revealing the complex regulation of TSHbeta expression and unexpectedly rapid response to light changes in the mammalian photoperiodic system.
(MeSH Terms)

Contact
Hiroki R. Ueda , RIKEN , Center for Developmental Biolog , Functional Genomics Unit
Contributors
Hiroki R. Ueda, Koh-hei Masumoto, Maki Ukai-Tadenuma, Takeya Kasukawa, Mamoru Nagano, Kenichiro D. Uno, Kaori Tsujino, Kazumasa Horikawa, Yasufumi Shigeyoshi


Dataset List of 9-Masumoto-Transcriptome

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
Download BDML
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# 498
Dataset Kind Quantitative data
Dataset Size 6.1 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 499
Dataset Kind Quantitative data
Dataset Size 6.1 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 500
Dataset Kind Quantitative data
Dataset Size 6.1 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 501
Dataset Kind Quantitative data
Dataset Size 6.1 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 502
Dataset Kind Quantitative data
Dataset Size 6.1 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 503
Dataset Kind Quantitative data
Dataset Size 6.1 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 504
Dataset Kind Quantitative data
Dataset Size 6.1 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 505
Dataset Kind Quantitative data
Dataset Size 6.1 MB
4D view
SSBD:OMERO
Download BDML
Download Image data