Summary of 79-Inomata-HeLaCell

SSBD:database
SSBD:database URL
Title
Intracellular distribution of hAK1 and hUB-3A delivered into HeLa cells.
Description
-
Relase date
2018-11-14
Updated date
-
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
8 ( Image datasets: 8, Quantitative data datasets: 0 )
Size of Datasets
6.0 MB ( Image datasets: 6.0 MB, Quantitative data datasets: 0 bytes )

Organism(s)
H. sapiens
Strain(s)
HeLa
Gene symbol(s)
hAK1, hUb-3A
Protein name(s)
NA

Datatype
intracellular structure
Molecular Function (MF)
Biological Process (BP)
cellular localization
Cellular Component (CC)
intracellular organelle
Biological Imaging Method
-
XYZ Scale
XY: 0.17297 micrometer/pixel, Z: NA
T scale
-

Image Acquisition
Experiment type
Other
Microscope type
ConfocalMicroscope
Acquisition mode
LaserScanningConfocalMicroscopy
Contrast method
Fluorescence
Microscope model
Olympus FluoView FV10i-DOC
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

Kohsuke Inomata, Hajime Kamoshida, Masaomi Ikari, Yutaka Ito, Takanori Kigawa (2017) Impact of cellular health conditions on the protein folding state in mammalian cells., Chemical communications (Cambridge, England), Volume 53, Number 81, pp. 11245-11248

Published in 2017 Oct 10

(Abstract) By using in-cell NMR experiments, we have demonstrated that the protein folding state in cells is significantly influenced by the cellular health conditions. hAK1 was denatured in cells under stressful culture conditions, while it remained functional and properly folded in cells continuously supplied with a fresh medium.
(MeSH Terms)

Contact
Kohsuke Inomata, Takanori Kigawa , RIKEN , Center for Biosystems Dynamics Research , Laboratory for Cellular Structural Biology
Contributors
Kohsuke Inomata, Hajime Kamoshida, Masaomi Ikari, Yutaka Ito, Takanori Kigawa


Dataset List of 79-Inomata-HeLaCell

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
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# 4043
Datast ID FigS1A
Dataset Kind Image data
Dataset Size 768.5 KB
4D view
SSBD:OMERO
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# 4044
Datast ID FigS1B
Dataset Kind Image data
Dataset Size 768.5 KB
4D view
SSBD:OMERO
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# 4045
Datast ID FigS1C
Dataset Kind Image data
Dataset Size 768.5 KB
4D view
SSBD:OMERO
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# 4046
Datast ID FigS1D
Dataset Kind Image data
Dataset Size 768.5 KB
4D view
SSBD:OMERO
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# 4047
Datast ID FigS2A
Dataset Kind Image data
Dataset Size 768.5 KB
4D view
SSBD:OMERO
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# 4048
Datast ID FigS2B
Dataset Kind Image data
Dataset Size 768.5 KB
4D view
SSBD:OMERO
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# 4049
Datast ID FigS2C
Dataset Kind Image data
Dataset Size 768.5 KB
4D view
SSBD:OMERO
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# 4050
Datast ID FigS2D
Dataset Kind Image data
Dataset Size 768.5 KB
4D view
SSBD:OMERO
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