SSBD:database

Detail of FigS1B

(Too many images for preview; see images in SSBD:OMERO Dataset)
Project
79-Inomata-HeLaCell
Title
Fluorescence image of intracellular distribution of Alexa488-labeled hAK1 in HeLa cells.
Description
NA
Release, Updated
2018-11-14
License
CC BY
Kind
Image data based on Experiment
File Formats
Data size
768.5 KB
Organism
H. sapiens ( NCBI:txid9606 )
Strain(s)
HeLa
Cell Line
-
Gene symbols
hAK1
Datatype
intracellular structure
Molecular Function (MF)
Biological Process (BP)
cellular localization ( GO:1902580 )
Cellular Component (CC)
intracellular organelle ( GO:0043229 )
Biological Imaging Method
XYZ Scale
XY: 0.17297 micrometer/pixel, Z: NA
T scale
-
Image Acquisition
Experiment type
Other
Microscope type
ConfocalMicroscope
Acquisition mode
LaserScanningConfocalMicroscopy
Contrast method
Fluorescence
Microscope model
Olympus FluoView FV10i-DOC
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
See details in Inomata et al. (2017) Chem Commun, 53(81): 11245-11248.
Related paper(s)

Kohsuke Inomata, Hajime Kamoshida, Masaomi Ikari, Yutaka Ito, Takanori Kigawa (2017) Impact of cellular health conditions on the protein folding state in mammalian cells., Chemical communications (Cambridge, England), Volume 53, Number 81, pp. 11245-11248

Published in 2017 Oct 10

(Abstract) By using in-cell NMR experiments, we have demonstrated that the protein folding state in cells is significantly influenced by the cellular health conditions. hAK1 was denatured in cells under stressful culture conditions, while it remained functional and properly folded in cells continuously supplied with a fresh medium.
(MeSH Terms)
Contact
Kohsuke Inomata, Takanori Kigawa , RIKEN , Center for Biosystems Dynamics Research , Laboratory for Cellular Structural Biology
Contributors
Kohsuke Inomata, Hajime Kamoshida, Masaomi Ikari, Yutaka Ito, Takanori Kigawa
OMERO Dataset
https://ssbd.riken.jp/omero/webclient/?show=dataset-867
OMERO Project
https://ssbd.riken.jp/omero/webclient/?show=project-157
Source
https://ssbd.riken.jp/data/79-Inomata-HeLaCell/source/FigS1B.zip (md5, 219.2 KB)