Summary of 253-Shimauchi-CalciumIonDyn

SSBD:database
SSBD:database URL
Title
Time courses images of Ca2+ responses in the TRPC inhibitor-expressing HEK293 cells or HeLa cells.
Description
-
Relase date
2022-11-30
Updated date
-
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
13 ( Image datasets: 13, Quantitative data datasets: 0 )
Size of Datasets
575.5 MB ( Image datasets: 575.5 MB, Quantitative data datasets: 0 bytes )

Organism(s)
Homo sapiens
Cell lines(s)
HeLa cell, HEK293
Gene symbol(s)
NA
Protein name(s)
TRPC3, TRPC3 inhibitors, TRPC6, TRPC7

Datatype
-
Molecular Function (MF)
Biological Process (BP)
response to calcium ion
Cellular Component (CC)
Biological Imaging Method
fluorescence microscopy
X scale
-
Y scale
-
Z scale
-
T scale
5 seconds of time interval

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

Tsukasa Shimauchi, Takuro Numaga-Tomita, Yuri Kato, Hiroyuki Morimoto, Kosuke Sakata, Ryosuke Matsukane, Akiyuki Nishimura, Kazuhiro Nishiyama, Atsushi Shibuta, Yutoku Horiuchi, Hitoshi Kurose, Sang Geon Kim, Yasuteru Urano, Takashi Ohshima, Motohiro Nishida (2022) A TRPC3/6 Channel Inhibitor Promotes Arteriogenesis after Hind-Limb Ischemia., Cells, Volume 11, Number 13

Published in 2022 Jun 27 (Electronic publication in June 27, 2022, midnight )

(Abstract) Retarded revascularization after progressive occlusion of large conductance arteries is a major cause of bad prognosis for peripheral artery disease (PAD). However, pharmacological treatment for PAD is still limited. We previously reported that suppression of transient receptor potential canonical (TRPC) 6 channel activity in vascular smooth muscle cells (VSMCs) facilitates VSMC differentiation without affecting proliferation and migration. In this study, we found that 1-benzilpiperadine derivative (1-BP), a selective inhibitor for TRPC3 and TRPC6 channel activities, induced VSMC differentiation. 1-BP-treated mice showed increased capillary arterialization and improvement of peripheral circulation and skeletal muscle mass after hind-limb ischemia (HLI) in mice. 1-BP had no additive effect on the facilitation of blood flow recovery after HLI in TRPC6-deficient mice, suggesting that suppression of TRPC6 underlies facilitation of the blood flow recovery by 1-BP. 1-BP also improved vascular nitric oxide bioavailability and blood flow recovery after HLI in hypercholesterolemic mice with endothelial dysfunction, suggesting the retrograde interaction from VSMCs to endothelium. These results suggest that 1-BP becomes a potential seed for PAD treatments that target vascular TRPC6 channels.
(MeSH Terms)

Contact
Motohiro Nishida , Kyushu University , Graduate School of Pharmaceutical Sciences
Contributors


Dataset List of 253-Shimauchi-CalciumIonDyn

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
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# 8807
Datast ID Fig2B_Clomiphene
Dataset Kind Image data
Dataset Size 19.9 MB
4D view
SSBD:OMERO
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# 8808
Datast ID Fig2B_CompoundB
Dataset Kind Image data
Dataset Size 19.9 MB
4D view
SSBD:OMERO
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# 8809
Datast ID Fig2B_Control
Dataset Kind Image data
Dataset Size 19.9 MB
4D view
SSBD:OMERO
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# 8810
Dataset Kind Image data
Dataset Size 19.9 MB
4D view
SSBD:OMERO
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# 8811
Datast ID Fig2B_Raloxiphene
Dataset Kind Image data
Dataset Size 19.9 MB
4D view
SSBD:OMERO
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# 8812
Datast ID Fig2B_Suloctidil
Dataset Kind Image data
Dataset Size 19.9 MB
4D view
SSBD:OMERO
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# 8813
Datast ID Fig2B_Tribenoside
Dataset Kind Image data
Dataset Size 19.9 MB
4D view
SSBD:OMERO
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# 8814
Datast ID Fig3A_DMSO
Dataset Kind Image data
Dataset Size 19.9 MB
4D view
SSBD:OMERO
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# 8815
Datast ID Fig3A_non-TRPC3
Dataset Kind Image data
Dataset Size 19.9 MB
4D view
SSBD:OMERO
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# 8816
Datast ID Fig4A_TRPC3
Dataset Kind Image data
Dataset Size 118.9 MB
4D view
SSBD:OMERO
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# 8817
Datast ID Fig4B_TRPC6
Dataset Kind Image data
Dataset Size 118.9 MB
4D view
SSBD:OMERO
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# 8818
Datast ID Fig4C_TRPC7
Dataset Kind Image data
Dataset Size 118.9 MB
4D view
SSBD:OMERO
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# 8819
Datast ID Fig4E_control
Dataset Kind Image data
Dataset Size 39.7 MB
4D view
SSBD:OMERO
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