Summary of 184-Tanaka-SpineDyn

SSBD:database
SSBD:database URL
Title
Time-lapse images of spine morphology and dynamics in cultured neurons of WT or cortactin knockout (KO) mice.
Description
-
Relase date
2022-03-31
Updated date
-
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
11 ( Image datasets: 11, Quantitative data datasets: 0 )
Size of Datasets
199.7 MB ( Image datasets: 199.7 MB, Quantitative data datasets: 0 bytes )

Organism(s)
Mus musculus
Gene symbol(s)
NA
Protein name(s)
actin, cortactin
Protein tag(s)
GFP, PAGFP
Reporter(s)
DsRed2

Datatype
-
Molecular Function (MF)
-
Biological Process (BP)
-
Cellular Component (CC)
dendritic spine
Biological Imaging Method
time lapse microscopy
X scale
0.082 micrometer/pixel, 0.103 micrometer/pixel
Y scale
0.082 micrometer/pixel, 0.103 micrometer/pixel
Z scale
0.5 micrometer/slice
T scale
15.18 sec per time interval, 10.23 sec per time interval, 10.09 sec per time interval, 18.58 sec per time interval, 18.56 sec per time interval, 20 minutes per time interval

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

Shinji Tanaka, Yasutaka Masuda, Akihiro Harada, Shigeo Okabe (2020) Impaired actin dynamics and suppression of Shank2-mediated spine enlargement in cortactin knockout mice., Microscopy (Oxford, England), Volume 69, Number 1, pp. 44-52

Published in 2020 Mar 9

(Abstract) Cortactin regulates actin polymerization and stabilizes branched actin network. In neurons, cortactin is enriched in dendritic spines that contain abundant actin polymers. To explore the function of cortactin in dendritic spines, we examined spine morphology and dynamics in cultured neurons taken from cortactin knockout (KO) mice. Histological analysis revealed that the density and morphology of dendritic spines were not significantly different between wild-type (WT) and cortactin KO neurons. Time-lapse imaging of hippocampal slice cultures showed that the extent of spine volume change was similar between WT and cortactin KO neurons. Despite little effect of cortactin deletion on spine morphology and dynamics, actin turnover in dendritic spines was accelerated in cortactin KO neurons. Furthermore, we detected a suppressive effect of cortactin KO on spine head size under the condition of excessive spine enlargement induced by overexpression of a prominent postsynaptic density protein Shank2. These results suggest that cortactin may have a role in maintaining actin organization by stabilizing actin filaments near the postsynaptic density.
(MeSH Terms)

Contact
Shigeo Okabe , University of Tokyo , Department of Cellular Neurobiology, Graduate School of Medicine
Contributors


Dataset List of 184-Tanaka-SpineDyn

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
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# 7360
Dataset Kind Image data
Dataset Size 49.1 MB
4D view
SSBD:OMERO
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# 7361
Dataset Kind Image data
Dataset Size 50.6 MB
4D view
SSBD:OMERO
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# 7362
Dataset Kind Image data
Dataset Size 69.1 MB
4D view
SSBD:OMERO
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# 7363
Dataset Kind Image data
Dataset Size 47.2 KB
4D view
SSBD:OMERO
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# 7364
Dataset Kind Image data
Dataset Size 47.1 KB
4D view
SSBD:OMERO
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# 7365
Dataset Kind Image data
Dataset Size 47.2 KB
4D view
SSBD:OMERO
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# 7366
Dataset Kind Image data
Dataset Size 47.2 KB
4D view
SSBD:OMERO
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# 7367
Dataset Kind Image data
Dataset Size 12.2 MB
4D view
SSBD:OMERO
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# 7368
Dataset Kind Image data
Dataset Size 3.2 MB
4D view
SSBD:OMERO
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# 7369
Dataset Kind Image data
Dataset Size 12.2 MB
4D view
SSBD:OMERO
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# 7370
Dataset Kind Image data
Dataset Size 3.2 MB
4D view
SSBD:OMERO
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