Summary of 17-Aoki-MolDyn

SSBD:database
SSBD:database URL
Title
-
Description
-
Relase date
2016-10-03
Updated date
2018-11-15
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
2 ( Image datasets: 2, Quantitative data datasets: 0 )
Size of Datasets
157.1 MB ( Image datasets: 157.1 MB, Quantitative data datasets: 0 bytes )

Organism(s)
R. norvegicus
Strain(s)
NRK-52E
Gene symbol(s)
NA
Protein name(s)
ERK

Datatype
cell dynamics
Molecular Function (MF)
Biological Process (BP)
cellular protein localization
Cellular Component (CC)
-
Biological Imaging Method
-
XYZ Scale
XY: 0.74 micrometer/pixel, Z: 0 micrometer/slice
T scale
3 minute for each time interval

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

Kazuhiro Aoki, Yuka Kumagai, Atsuro Sakurai, Naoki Komatsu, Yoshihisa Fujita, Clara Shionyu, Michiyuki Matsuda (2013) Stochastic ERK activation induced by noise and cell-to-cell propagation regulates cell density-dependent proliferation., Molecular cell, Volume 52, Number 4, pp. 529-40

Published in 2013 Nov 21 (Electronic publication in Oct. 17, 2013, midnight )

(Abstract) The extracellular signal-regulated kinase (ERK) plays a central role in the signaling cascades of cell growth. Here, we show that stochastic ERK activity pulses regulate cell proliferation rates in a cell density-dependent manner. A fluorescence resonance energy transfer (FRET) biosensor revealed that stochastic ERK activity pulses fired spontaneously or propagated from adjacent cells. Frequency, but not amplitude, of ERK activity pulses exhibited a bell-shaped response to the cell density and correlated with cell proliferation rates. Consistently, synthetic ERK activity pulses generated by a light-switchable CRaf protein accelerated cell proliferation. A mathematical model clarified that 80% and 20% of ERK activity pulses are generated by the noise and cell-to-cell propagation, respectively. Finally, RNA sequencing analysis of cells subjected to the synthetic ERK activity pulses suggested the involvement of serum responsive factor (SRF) transcription factors in the gene expression driven by the ERK activity pulses.
(MeSH Terms)

Contact
Kazuhiro Aoki , Kyoto University , Graduate School of Medicine , Imaging Platform for Spatio-Temporal Information
Contributors
Kazuhiro Aoki, Yuka Kumagai, Atsuro Sakurai, Naoki Komatsu, Yoshihisa Fujita, Clara Shionyu, Michiyuki Matsuda


Dataset List of 17-Aoki-MolDyn

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
Download BDML
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# 791
Datast ID sample1
Dataset Kind Image data
Dataset Size 25.7 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 792
Datast ID sample2
Dataset Kind Image data
Dataset Size 131.4 MB
4D view
SSBD:OMERO
Download BDML
Download Image data