Detail of sample2
Project
Title
Time-lapse fluorescence microscopy images of ERK activity in rat kidney epithelial (NRK-52E) cells obtained from in vivo experiments (total about 24 hour)
Description
NA
Release, Updated
2016-10-03,
2018-11-15
2018-11-15
License
CC BY
Kind
Image data
based on Experiment
File Formats
Data size
131.4 MB
Protein names
ERK
Datatype
cell dynamics
Molecular Function (MF)
Biological Process (BP)
cellular protein localization
(
GO:0034613
)
Cellular Component (CC)
-
Biological Imaging Method
XYZ Scale
XY: 0.74 micrometer/pixel, Z: 0 micrometer/slice
T scale
3 minute for each time interval
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
See details in Aoki et al. (2013) Molecular Cell, 52(4): 529-540
Related paper(s)
Kazuhiro Aoki, Yuka Kumagai, Atsuro Sakurai, Naoki Komatsu, Yoshihisa Fujita, Clara Shionyu, Michiyuki Matsuda (2013) Stochastic ERK activation induced by noise and cell-to-cell propagation regulates cell density-dependent proliferation., Molecular cell, Volume 52, Number 4, pp. 529-40
Published in 2013 Nov 21 (Electronic publication in Oct. 17, 2013, midnight )
- doi: 10.1016/j.molcel.2013.09.015
-
pmid: 24140422
(Abstract) The extracellular signal-regulated kinase (ERK) plays a central role in the signaling cascades of cell growth. Here, we show that stochastic ERK activity pulses regulate cell proliferation rates in a cell density-dependent manner. A fluorescence resonance energy transfer (FRET) biosensor revealed that stochastic ERK activity pulses fired spontaneously or propagated from adjacent cells. Frequency, but not amplitude, of ERK activity pulses exhibited a bell-shaped response to the cell density and correlated with cell proliferation rates. Consistently, synthetic ERK activity pulses generated by a light-switchable CRaf protein accelerated cell proliferation. A mathematical model clarified that 80% and 20% of ERK activity pulses are generated by the noise and cell-to-cell propagation, respectively. Finally, RNA sequencing analysis of cells subjected to the synthetic ERK activity pulses suggested the involvement of serum responsive factor (SRF) transcription factors in the gene expression driven by the ERK activity pulses.(MeSH Terms)
- Algorithms
- Animals
- Benzamides/pharmacology
- Cell Communication
- Cell Count
- Cell Line
- Cell Proliferation[major]
- Enzyme Activation
- Extracellular Signal-Regulated MAP Kinases/antagonists & inhibitors
- Extracellular Signal-Regulated MAP Kinases/metabolism[major]
- Gene Expression Regulation
- Humans
- Imidazoles/pharmacology
- Kinetics
- MAP Kinase Signaling System[major]
- Models, Biological
- Promoter Regions, Genetic
- Rats
- Sequence Analysis, RNA
- Stochastic Processes
- Time-Lapse Imaging
- raf Kinases/antagonists & inhibitors
- raf Kinases/metabolism
Contact
Kazuhiro Aoki
, Kyoto University
, Graduate School of Medicine
, Imaging Platform for Spatio-Temporal Information
Contributors
Kazuhiro Aoki, Yuka Kumagai, Atsuro Sakurai, Naoki Komatsu, Yoshihisa Fujita, Clara Shionyu, Michiyuki Matsuda
OMERO Dataset
OMERO Project
Source