SSBD:database

Keishi Kishimoto, Masaru Tamura, Michiru Nishita, Yasuhiro Minami, Akira Yamaoka, Takaya Abe, Mayo Shigeta, Mitsuru Morimoto (2018) Synchronized mesenchymal cell polarization and differentiation shape the formation of the murine trachea and esophagus., Nature communications, Volume 9, Number 1, pp. 2816

Published in 2018 Jul 19 (Electronic publication in July 19, 2018, midnight )

• doi: 10.1038/s41467-018-05189-2
• pmid: 30026494
  

(Abstract) Tube morphogenesis is essential for internal-organ development, yet the mechanisms regulating tube shape remain unknown. Here, we show that different mechanisms regulate the length and diameter of the murine trachea. First, we found that trachea development progresses via sequential elongation and expansion processes. This starts with a synchronized radial polarization of smooth muscle (SM) progenitor cells with inward Golgi-apparatus displacement regulates tube elongation, controlled by mesenchymal Wnt5a-Ror2 signaling. This radial polarization directs SM progenitor cell migration toward the epithelium, and the resulting subepithelial morphogenesis supports tube elongation to the anteroposterior axis. This radial polarization also regulates esophageal elongation. Subsequently, cartilage development helps expand the tube diameter, which drives epithelial-cell reshaping to determine the optimal lumen shape for efficient respiration. These findings suggest a strategy in which straight-organ tubulogenesis is driven by subepithelial cell polarization and ring cartilage development.

• Actin Cytoskeleton/metabolism
• Actin Cytoskeleton/ultrastructure
• Animals
• Cartilage/cytology
• Cartilage/growth & development
• Cartilage/metabolism[major]
• Cell Differentiation
• Cell Polarity
• Embryo, Mammalian
• Esophagus/cytology
• Esophagus/growth & development
• Esophagus/metabolism[major]
• Female
• Forkhead Transcription Factors/genetics
• Forkhead Transcription Factors/metabolism
• Gene Expression Regulation, Developmental
• Golgi Apparatus/metabolism
• Golgi Apparatus/ultrastructure
• Male
• Mesenchymal Stem Cells/cytology
• Mesenchymal Stem Cells/metabolism
• Mice
• Mice, Transgenic
• Morphogenesis/genetics[major]
• Muscle, Smooth/cytology
• Muscle, Smooth/metabolism[major]
• Myocytes, Smooth Muscle/cytology
• Myocytes, Smooth Muscle/metabolism[major]
• Nerve Tissue Proteins/genetics
• Nerve Tissue Proteins/metabolism
• Receptor Tyrosine Kinase-like Orphan Receptors/genetics
• Receptor Tyrosine Kinase-like Orphan Receptors/metabolism
• SOX9 Transcription Factor/genetics
• SOX9 Transcription Factor/metabolism
• Signal Transduction
• Trachea/cytology
• Trachea/growth & development
• Trachea/metabolism[major]
• Wnt-5a Protein/genetics
• Wnt-5a Protein/metabolism